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题名: Capillary zone electrophoresis investigation of the interaction between heparin and granulocyte-colony stimulating factor
作者: Liang, AY;  He, XY;  Du, YG;  Wang, KY;  Fung, YS;  Lin, BC
关键词: capillary zone electrophoresis ;  granulocyte-colony stimulating factor ;  heparin
刊名: ELECTROPHORESIS
发表日期: 2004-03-01
DOI: 10.1002/elps.200305728
卷: 25, 期:6, 页:870-875
收录类别: SCI
文章类型: Article
项目归属: 1807,1805
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The interaction between standard heparin, low-molecular-weight heparin (LMWH), and granulocyte-colony stimulating factor (G-CSF) was studied by capillary zone electrophoresis. Both qualitative and quantitative characterizations of the heparin-protein binding were determined. The binding constants of the two different groups of heparins with G-CSF, calculated from the Scatchard plot by regression, were 4.805 x 10(5) m(-1) and 4.579 x 10(5) m(-1), respectively. The two binding constants measured are of the same order of magnitude at 10(5) m(-1), indicating that LMWH contains most of the functional groups bound to G-CSF by standard heparin.
关键词[WOS]: FIBROBLAST-GROWTH-FACTOR ;  HUMAN SERUM-ALBUMIN ;  AMYLOID-P COMPONENT ;  ANTITHROMBIN-BINDING ;  DRUG ENANTIOMERS ;  DOMAIN ;  DNA ;  PEPTIDES ;  SEQUENCE ;  SITE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000220347900014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81101
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Shanghai Acad Life Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China
3.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Liang, AY,He, XY,Du, YG,et al. Capillary zone electrophoresis investigation of the interaction between heparin and granulocyte-colony stimulating factor[J]. ELECTROPHORESIS,2004,25(6):870-875.
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