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题名: Integrated lectin affinity microfluidic chip for glycoform separation
作者: Mao, XL;  Luo, Y;  Dai, ZP;  Wang, KY;  Du, YG;  Lin, BC
刊名: ANALYTICAL CHEMISTRY
发表日期: 2004-12-01
DOI: 10.1021/ac049270g
卷: 76, 期:23, 页:6941-6947
收录类别: SCI
文章类型: Article
项目归属: 1807,1805
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Lectin affinity chromatography was miniaturized into a microfluidic format, which results in improvement of performance, as compared to the conventional method. A lectin affinity monolith column was prepared in the microchannel of a microfluidic chip. The porous monolith was fabricated by UV-initiated polymerization of ethylene dimethacrylate (EDMA) and glycidyl methacrylate (GMA) in the presence of porogeneities, followed by immobilization of pisum sativum agglutinin (PSA) on the monolith matrix. Using electroosmosis as the driven force, lectin affinity chromatographies of three kinds of glycoprotein, turkey ovalbumin (TO), chicken ovalbumin (CO), and ovomucoid (OM), were carried out on the microfluidic system. All the glycoproteins were successfully separated into several fractions with different affinities toward the immobilized PSA. The integrated system reduces the time required for the lectin affinity chromatography reaction to similar to3%, thus, the overall analysis time from 4 h to 400 s. Only 300 pg of glycoprotein is required for the whole separation process. Moreover, troublesome operations for lectin affinity chromatography are simplified.
关键词[WOS]: LINKED SUGAR CHAINS ;  CAPILLARY-ELECTROPHORESIS ;  GLYCOPROTEIN GLYCOFORMS ;  LIQUID-CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  BEADS ;  CARCINOMA ;  DIGESTION ;  CHANNELS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000225535300021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81105
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Beijing 100864, Peoples R China
2.Chinese Acad Sci, Shanghai Acad Life Sci, Inst Biochem & Cell Biol, Beijing 100864, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China

Recommended Citation:
Mao, XL,Luo, Y,Dai, ZP,et al. Integrated lectin affinity microfluidic chip for glycoform separation[J]. ANALYTICAL CHEMISTRY,2004,76(23):6941-6947.
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