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题名: Pressurized electrochromatography coupled with electrospray ionization mass spectrometry for analysis of peptides and proteins
作者: Liang, Z;  Duan, JC;  Zhang, UH;  Zhang, WB;  Zhang, YK;  Yan, C
刊名: ANALYTICAL CHEMISTRY
发表日期: 2004-12-01
DOI: 10.1021/ac0491410
卷: 76, 期:23, 页:6935-6940
收录类别: SCI
文章类型: Article
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Pressurized capillary electrochromatography (pCEC) was coupled with electrospray ionization mass spectrometry (ESI-MS) using a coaxial sheath liquid interface. It was used for separation and analysis of peptides and proteins. The effects of organic modifier and applied voltage on separation were investigated, and the effects of pH value of the mobile phase and the concentration of the electrolyte on ESI-MS signal were investigated. The resolution and detection sensitivity with different separation methods (pCEC, capillary high-performance liquid chromatography) coupled on-line with mass spectrometry were compared for the separation of a peptide mixture. To evaluate the feasibility and reliability of the experimental setup of the system, tryptic digests of cytochrome c and modified protein as real samples were analyzed by using pCEC-ESI-MS.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  CAPILLARY ELECTROCHROMATOGRAPHY ;  SEPARATION ;  INTERFACE ;  COLUMNS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000225535300020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81141
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
2.Unimicro Technol Inc, Pleasanton, CA 94566 USA

Recommended Citation:
Liang, Z,Duan, JC,Zhang, UH,et al. Pressurized electrochromatography coupled with electrospray ionization mass spectrometry for analysis of peptides and proteins[J]. ANALYTICAL CHEMISTRY,2004,76(23):6935-6940.
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