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Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe
Jin, SY; Xu, ZC; Chen, JP; Liang, XM; Wu, YN; Qian, XH
KeywordAcetylcholinesterase Fluorescence Probe Flow-injection Analysis Organophosphate Pesticides Carbamate Pesticides Vegetable Juices
Source PublicationANALYTICA CHIMICA ACTA
2004-10-04
DOI10.1016/j.aca.2004.05.030
Volume523Issue:1Pages:117-123
Indexed BySCI
SubtypeArticle
Funding Project103
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordFLOW-INJECTION ANALYSIS ; IMMOBILIZED ACETYLCHOLINESTERASE ; BIOSENSOR ; PARAOXON ; CHOLINESTERASE ; SENSOR ; INSECTICIDES ; EMISSION ; REACTOR ; SAMPLES
AbstractA flow injection system for the determination of organophosphate and carbamate pesticides is described. A sensitive fluorescence probe was synthesized and used as the pH indicator to detect the inhibition of the enzyme acetylcholinesterase (ACNE). The percentage inhibition of enzyme activity is correlated to the pesticide concentration. Several parameters influencing the performance of the system are discussed. The detection limits of 3.5, 50, 12 and 25 mug/l for carbofuran, carbaryl, paraoxon and dichlorvos, in pure water, respectively were achieved with an incubation time of 10 min. A complete cycle of analysis, including incubation time, took 14 min. The detection system has been applied to the determination of carbofuran in spiked vegetable juices (Chinese cabbage and cole), achieving recovery values between 93.2 and 107% for Chinese cabbage juice and 108 and 118% for cole juice at the different concentration levels assayed. (C) 2004 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000224039700017
Citation statistics
Cited Times:70[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/81215
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China
2.Dalian Univ Technol, Dept Fine Chem Engn, Dalian 116012, Peoples R China
3.Natl Inst Nutr & Food Safety, Chinese Ctr Dis Control & Prevent, Beijing 100050, Peoples R China
Recommended Citation
GB/T 7714
Jin, SY,Xu, ZC,Chen, JP,et al. Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe[J]. ANALYTICA CHIMICA ACTA,2004,523(1):117-123.
APA Jin, SY,Xu, ZC,Chen, JP,Liang, XM,Wu, YN,&Qian, XH.(2004).Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe.ANALYTICA CHIMICA ACTA,523(1),117-123.
MLA Jin, SY,et al."Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe".ANALYTICA CHIMICA ACTA 523.1(2004):117-123.
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