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Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe
Jin, SY; Xu, ZC; Chen, JP; Liang, XM; Wu, YN; Qian, XH
关键词Acetylcholinesterase Fluorescence Probe Flow-injection Analysis Organophosphate Pesticides Carbamate Pesticides Vegetable Juices
刊名ANALYTICA CHIMICA ACTA
2004-10-04
DOI10.1016/j.aca.2004.05.030
523期:1页:117-123
收录类别SCI
文章类型Article
项目归属103
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]FLOW-INJECTION ANALYSIS ; IMMOBILIZED ACETYLCHOLINESTERASE ; BIOSENSOR ; PARAOXON ; CHOLINESTERASE ; SENSOR ; INSECTICIDES ; EMISSION ; REACTOR ; SAMPLES
英文摘要A flow injection system for the determination of organophosphate and carbamate pesticides is described. A sensitive fluorescence probe was synthesized and used as the pH indicator to detect the inhibition of the enzyme acetylcholinesterase (ACNE). The percentage inhibition of enzyme activity is correlated to the pesticide concentration. Several parameters influencing the performance of the system are discussed. The detection limits of 3.5, 50, 12 and 25 mug/l for carbofuran, carbaryl, paraoxon and dichlorvos, in pure water, respectively were achieved with an incubation time of 10 min. A complete cycle of analysis, including incubation time, took 14 min. The detection system has been applied to the determination of carbofuran in spiked vegetable juices (Chinese cabbage and cole), achieving recovery values between 93.2 and 107% for Chinese cabbage juice and 108 and 118% for cole juice at the different concentration levels assayed. (C) 2004 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000224039700017
引用统计
被引频次:66[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/81215
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China
2.Dalian Univ Technol, Dept Fine Chem Engn, Dalian 116012, Peoples R China
3.Natl Inst Nutr & Food Safety, Chinese Ctr Dis Control & Prevent, Beijing 100050, Peoples R China
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GB/T 7714
Jin, SY,Xu, ZC,Chen, JP,et al. Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe[J]. ANALYTICA CHIMICA ACTA,2004,523(1):117-123.
APA Jin, SY,Xu, ZC,Chen, JP,Liang, XM,Wu, YN,&Qian, XH.(2004).Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe.ANALYTICA CHIMICA ACTA,523(1),117-123.
MLA Jin, SY,et al."Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe".ANALYTICA CHIMICA ACTA 523.1(2004):117-123.
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