中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Determination and identification of isoflavonoids in Radix astragali by matrix solid-phase dispersion extraction and high-performance liquid chromatography with photodiode array and mass spectrometric detection
作者: Xiao, HB;  Krucker, M;  Albert, K;  Liang, XM
关键词: Radix astragali ;  matrix solid-phase dispersion ;  extraction methods ;  plant materials ;  Astragalus spp. ;  isoflavonoids ;  flavonoids
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2004-04-02
DOI: 10.1016/j.chroma.2003.09.032
卷: 1032, 期:1-2, 页:117-124
收录类别: ISTP ;  SCI
文章类型: Article
项目归属: 1804
产权排名: 2;4
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The isoflavonoids in Radix astragali were determined and identified by HPLC-photodiode array detection-MS after extraction employing matrix solid-phase dispersion (MSPD). As a new sample preparation method for R. astragali, the MSPD procedure was optimized, validated and compared with conventional methods including ultrasonic and Soxhlet extraction. The amounts of two major components in this herb, formononetin (6) and ononin (2), were determined based on their authentic standards. Four major isoflavonoids, formononetin (6), ononin (2), calycosin (5) and its glycoside (1), and three minor isoflavonoids, (6aR,11aR)-3-hydroxy-9, 10-dimethoxypterocarpan (7), its glycoside (3), and (3R)-7,2'-dihydroxy-3',4'-dimethoxyisoflavone-7-O-beta-D-glycoside (4), were identified based on their characteristic two-band UV spectra and [M + H](+), [aglycone + H](+) and [A1 + H](+) ions, etc. The combined MSPD and HPLC-DAD-MS method was suitable for quantitative and qualitative determination of the isoflavonoids in R. astragali. (C) 2003 Elsevier B.V. All rights reserved.
关键词[WOS]: MAGNETIC-RESONANCE-SPECTROSCOPY ;  NATURAL-PRODUCTS ;  COMBINATION ;  FLAVONOIDS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000220121200018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81493
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2004GH2X69S189.pdf(218KB)----开放获取--View Download

作者单位: 1.Univ Tubingen, Inst Organ Chem, D-72076 Tubingen, Germany
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Xiao, HB,Krucker, M,Albert, K,et al. Determination and identification of isoflavonoids in Radix astragali by matrix solid-phase dispersion extraction and high-performance liquid chromatography with photodiode array and mass spectrometric detection[J]. JOURNAL OF CHROMATOGRAPHY A,2004,1032(1-2):117-124.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Xiao, HB]'s Articles
 [Krucker, M]'s Articles
 [Albert, K]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Xiao, HB]‘s Articles
 [Krucker, M]‘s Articles
 [Albert, K]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2004GH2X69S189.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace