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题名: Detection of K-ras exon 1 mutations by constant denaturant capillary electrophoresis
作者: Zhao, CX;  Xu, GW;  Shi, XZ;  Ma, JM;  Lu, S;  Yang, Q
关键词: constant denaturant capillary electrophoresis ;  K-ras gene ;  mutation detection
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2004-10-01
DOI: 10.1002/bmc.353
卷: 18, 期:8, 页:538-541
收录类别: SCI
文章类型: Article
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology ;  Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Pharmacology & Pharmacy
英文摘要: Among various mutation detection methods, constant denaturant capillary electrophoresis (CDCE) is one of the most common techniques for rapid identification of known or unknown mutations. In this report, a CDCE analysis method with homemade linear polyacrylamide (LPA) kit was developed on ABI 310 genetic analyzer, the effect and relationship of various denaturing factors in CDCE analysis were investigated and K-ras gene mutations of 31 coloerctal cancer patients were detected. Results indicate that, with the increase of chemical danaturant concentration, the optimum temperature was lowered, and when the concentration of urea (formamide) was higher than 7 M (40%), the homoduplex and heteroduplex of mutant samples were separated with difficulty. Detection results of K-ras gene in colorectal samples indicated that mutations were present in eight (26%) of 31 patients; most mutations were localized in codon 12, which is thought to be a critical step and plays an important role in human colorectal carcinogenesisas. Copyright (C) 2004 John Wiley Sons, Ltd.
关键词[WOS]: GENE ;  DNA ;  CDCE ;  SEPARATION ;  P53
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000224314500008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81583
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Dalian Med Univ, Secondary Affiliated Hosp, Dalian 116027, Peoples R China

Recommended Citation:
Zhao, CX,Xu, GW,Shi, XZ,et al. Detection of K-ras exon 1 mutations by constant denaturant capillary electrophoresis[J]. BIOMEDICAL CHROMATOGRAPHY,2004,18(8):538-541.
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