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Detection of K-ras exon 1 mutations by constant denaturant capillary electrophoresis
Zhao, CX; Xu, GW; Shi, XZ; Ma, JM; Lu, S; Yang, Q
关键词Constant Denaturant Capillary Electrophoresis K-ras Gene Mutation Detection
刊名BIOMEDICAL CHROMATOGRAPHY
2004-10-01
DOI10.1002/bmc.353
18期:8页:538-541
收录类别SCI
文章类型Article
项目归属1808
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]GENE ; DNA ; CDCE ; SEPARATION ; P53
英文摘要Among various mutation detection methods, constant denaturant capillary electrophoresis (CDCE) is one of the most common techniques for rapid identification of known or unknown mutations. In this report, a CDCE analysis method with homemade linear polyacrylamide (LPA) kit was developed on ABI 310 genetic analyzer, the effect and relationship of various denaturing factors in CDCE analysis were investigated and K-ras gene mutations of 31 coloerctal cancer patients were detected. Results indicate that, with the increase of chemical danaturant concentration, the optimum temperature was lowered, and when the concentration of urea (formamide) was higher than 7 M (40%), the homoduplex and heteroduplex of mutant samples were separated with difficulty. Detection results of K-ras gene in colorectal samples indicated that mutations were present in eight (26%) of 31 patients; most mutations were localized in codon 12, which is thought to be a critical step and plays an important role in human colorectal carcinogenesisas. Copyright (C) 2004 John Wiley Sons, Ltd.
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WOS记录号WOS:000224314500008
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/81583
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Dalian Med Univ, Secondary Affiliated Hosp, Dalian 116027, Peoples R China
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Zhao, CX,Xu, GW,Shi, XZ,et al. Detection of K-ras exon 1 mutations by constant denaturant capillary electrophoresis[J]. BIOMEDICAL CHROMATOGRAPHY,2004,18(8):538-541.
APA Zhao, CX,Xu, GW,Shi, XZ,Ma, JM,Lu, S,&Yang, Q.(2004).Detection of K-ras exon 1 mutations by constant denaturant capillary electrophoresis.BIOMEDICAL CHROMATOGRAPHY,18(8),538-541.
MLA Zhao, CX,et al."Detection of K-ras exon 1 mutations by constant denaturant capillary electrophoresis".BIOMEDICAL CHROMATOGRAPHY 18.8(2004):538-541.
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