DICP OpenIR
Applications of homemade kit in mutation detection of genes
Zhao, CX; Xu, GW; Shi, XZ; Ma, JM; Zhang, Y; Lu, S; Yang, Q
关键词Single Nucleotide Polymorphism (Snp) Single-strand Conformation Pollymorphism (Sscp) Heteroduplex Analysis (Ha) Snapshot Linear Polyacrylamide (Lpa) Polydimethylacrylamide (Pdma)
刊名SCIENCE IN CHINA SERIES B-CHEMISTRY
2004-04-01
DOI10.1360/03yb0044
47期:2页:166-172
收录类别SCI
文章类型Article
项目归属1808
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]STRAND CONFORMATION POLYMORPHISM ; SINGLE NUCLEOTIDE POLYMORPHISMS ; CAPILLARY-ELECTROPHORESIS ; GEL-ELECTROPHORESIS ; DNA SEPARATION ; RESOLUTION ; POLYMERS
英文摘要Several methods of mutation detection, such as single-strand conformation polymorphism (SSCP), tandem SSCP/heteroduplex analysis and SNaPshot analysis were developed using homemade kit on ABI 310 genetic analyzer, and were successfully applied to mutation detection of 31 colorectal tumor samples. The sieving capability of homemade kit and commercial kit were compared, results demonstrate that homemade kit has higher resolution and shorter analysis time. In clinical tumor samples, 26% K-ras (exon 1) and 24% p53 (exons 7-8) were found to have mutations, and all mutations were single point variations. A majority of mutations occurred in one gene, only 1 tumor contained alterations in the two genes, which indicates that development of colorectal cancer lies on alternate pathways, and may correlate with different gene mutations.
语种英语
原文出处查看原文
WOS记录号WOS:000221119100012
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/81587
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Cell Lab, Dalian 116023, Peoples R China
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GB/T 7714
Zhao, CX,Xu, GW,Shi, XZ,et al. Applications of homemade kit in mutation detection of genes[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2004,47(2):166-172.
APA Zhao, CX.,Xu, GW.,Shi, XZ.,Ma, JM.,Zhang, Y.,...&Yang, Q.(2004).Applications of homemade kit in mutation detection of genes.SCIENCE IN CHINA SERIES B-CHEMISTRY,47(2),166-172.
MLA Zhao, CX,et al."Applications of homemade kit in mutation detection of genes".SCIENCE IN CHINA SERIES B-CHEMISTRY 47.2(2004):166-172.
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