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题名: Applications of homemade kit in mutation detection of genes
作者: Zhao, CX;  Xu, GW;  Shi, XZ;  Ma, JM;  Zhang, Y;  Lu, S;  Yang, Q
关键词: single nucleotide polymorphism (SNP) ;  single-strand conformation pollymorphism (SSCP) ;  heteroduplex analysis (HA) ;  SNaPshot ;  linear polyacrylamide (LPA) ;  polydimethylacrylamide (PDMA)
刊名: SCIENCE IN CHINA SERIES B-CHEMISTRY
发表日期: 2004-04-01
DOI: 10.1360/03yb0044
卷: 47, 期:2, 页:166-172
收录类别: SCI
文章类型: Article
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Several methods of mutation detection, such as single-strand conformation polymorphism (SSCP), tandem SSCP/heteroduplex analysis and SNaPshot analysis were developed using homemade kit on ABI 310 genetic analyzer, and were successfully applied to mutation detection of 31 colorectal tumor samples. The sieving capability of homemade kit and commercial kit were compared, results demonstrate that homemade kit has higher resolution and shorter analysis time. In clinical tumor samples, 26% K-ras (exon 1) and 24% p53 (exons 7-8) were found to have mutations, and all mutations were single point variations. A majority of mutations occurred in one gene, only 1 tumor contained alterations in the two genes, which indicates that development of colorectal cancer lies on alternate pathways, and may correlate with different gene mutations.
关键词[WOS]: STRAND CONFORMATION POLYMORPHISM ;  SINGLE NUCLEOTIDE POLYMORPHISMS ;  CAPILLARY-ELECTROPHORESIS ;  GEL-ELECTROPHORESIS ;  DNA SEPARATION ;  RESOLUTION ;  POLYMERS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000221119100012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81587
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Cell Lab, Dalian 116023, Peoples R China

Recommended Citation:
Zhao, CX,Xu, GW,Shi, XZ,et al. Applications of homemade kit in mutation detection of genes[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2004,47(2):166-172.
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