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Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking
Yao, QH; Mei, SR; Weng, QF; Zhang, PD; Yang, Q; Wu, CY; Xu, GW
关键词8-hydroxy-2 '-deoxyguanosine Urine Cigarette Smoking Capillary Electrophoresis End-column Amperometric Detection
刊名TALANTA
2004-06-17
DOI10.1016/j.talanta.2003.12.024
63期:3页:617-623
收录类别SCI
文章类型Article
项目归属1808
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; CAPILLARY-ZONE-ELECTROPHORESIS ; COLUMN AMPEROMETRIC DETECTION ; MICRODISK ARRAY ELECTRODE ; SOLID-PHASE EXTRACTION ; DYNAMIC PH JUNCTION ; ELECTROCHEMICAL DETECTION ; GAS-CHROMATOGRAPHY ; CANCER-PATIENTS ; 8-HYDROXYDEOXYGUANOSINE
英文摘要8-hydroxy-2'-deoxyguanosine (8OHdG) has been widely used as a biomarker of oxidative DNA damage in both animal models and human studies. To evaluate the effect of cigarette smoking on oxidative stress, we studied the levels of urinary 8OHdG from smokers and non-smokers and investigated the association with cigarette smoking. The urinary 8OHdG concentrations were determinated by capillary electrophoresis with end-column amprometric detection (CE-AD) after a single-step solid phase extraction (SPE), and then quantitatively expressed as a function of creatinine excretion. To increase the concentration sensitivity, a dynamic pH junction was used and the focusing effect was obvious when using 30 mM phosphate (pH 6.50) as sample matrix. The limit of detection is 4.3 nM (signal-to-noise ratio S/N = 3). The relative standard deviation (R.S.D.) was 1.1% for peak current, and 2.3% for migration time. Based on the selected CE-AD method, it was found that the mean value of urinary 8OHdG levels in the smokers significantly higher than that in non-smokers (31.4 +/- 18.9 nM versus 14.4 +/- 7.6 nM, P = 0.0004; 23.5 +/- 21.3 mug g(-1) creatinine versus 12.6 +/- 13.2 mug g(-1) creatinine, P = 0.028). (C) 2004 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000221735500018
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被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/81591
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Wuhan Univ, Coll Chem, Wuhan 430072, Peoples R China
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Yao, QH,Mei, SR,Weng, QF,et al. Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking[J]. TALANTA,2004,63(3):617-623.
APA Yao, QH.,Mei, SR.,Weng, QF.,Zhang, PD.,Yang, Q.,...&Xu, GW.(2004).Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking.TALANTA,63(3),617-623.
MLA Yao, QH,et al."Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking".TALANTA 63.3(2004):617-623.
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