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题名: Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking
作者: Yao, QH;  Mei, SR;  Weng, QF;  Zhang, PD;  Yang, Q;  Wu, CY;  Xu, GW
关键词: 8-hydroxy-2 '-deoxyguanosine ;  urine ;  cigarette smoking ;  capillary electrophoresis ;  end-column amperometric detection
刊名: TALANTA
发表日期: 2004-06-17
DOI: 10.1016/j.talanta.2003.12.024
卷: 63, 期:3, 页:617-623
收录类别: SCI
文章类型: Article
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: 8-hydroxy-2'-deoxyguanosine (8OHdG) has been widely used as a biomarker of oxidative DNA damage in both animal models and human studies. To evaluate the effect of cigarette smoking on oxidative stress, we studied the levels of urinary 8OHdG from smokers and non-smokers and investigated the association with cigarette smoking. The urinary 8OHdG concentrations were determinated by capillary electrophoresis with end-column amprometric detection (CE-AD) after a single-step solid phase extraction (SPE), and then quantitatively expressed as a function of creatinine excretion. To increase the concentration sensitivity, a dynamic pH junction was used and the focusing effect was obvious when using 30 mM phosphate (pH 6.50) as sample matrix. The limit of detection is 4.3 nM (signal-to-noise ratio S/N = 3). The relative standard deviation (R.S.D.) was 1.1% for peak current, and 2.3% for migration time. Based on the selected CE-AD method, it was found that the mean value of urinary 8OHdG levels in the smokers significantly higher than that in non-smokers (31.4 +/- 18.9 nM versus 14.4 +/- 7.6 nM, P = 0.0004; 23.5 +/- 21.3 mug g(-1) creatinine versus 12.6 +/- 13.2 mug g(-1) creatinine, P = 0.028). (C) 2004 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  CAPILLARY-ZONE-ELECTROPHORESIS ;  COLUMN AMPEROMETRIC DETECTION ;  MICRODISK ARRAY ELECTRODE ;  SOLID-PHASE EXTRACTION ;  DYNAMIC PH JUNCTION ;  ELECTROCHEMICAL DETECTION ;  GAS-CHROMATOGRAPHY ;  CANCER-PATIENTS ;  8-HYDROXYDEOXYGUANOSINE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000221735500018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81591
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Wuhan Univ, Coll Chem, Wuhan 430072, Peoples R China

Recommended Citation:
Yao, QH,Mei, SR,Weng, QF,et al. Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking[J]. TALANTA,2004,63(3):617-623.
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