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Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking
Yao, QH; Mei, SR; Weng, QF; Zhang, PD; Yang, Q; Wu, CY; Xu, GW
Keyword8-hydroxy-2 '-deoxyguanosine Urine Cigarette Smoking Capillary Electrophoresis End-column Amperometric Detection
Source PublicationTALANTA
2004-06-17
DOI10.1016/j.talanta.2003.12.024
Volume63Issue:3Pages:617-623
Indexed BySCI
SubtypeArticle
Funding Project1808
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; CAPILLARY-ZONE-ELECTROPHORESIS ; COLUMN AMPEROMETRIC DETECTION ; MICRODISK ARRAY ELECTRODE ; SOLID-PHASE EXTRACTION ; DYNAMIC PH JUNCTION ; ELECTROCHEMICAL DETECTION ; GAS-CHROMATOGRAPHY ; CANCER-PATIENTS ; 8-HYDROXYDEOXYGUANOSINE
Abstract8-hydroxy-2'-deoxyguanosine (8OHdG) has been widely used as a biomarker of oxidative DNA damage in both animal models and human studies. To evaluate the effect of cigarette smoking on oxidative stress, we studied the levels of urinary 8OHdG from smokers and non-smokers and investigated the association with cigarette smoking. The urinary 8OHdG concentrations were determinated by capillary electrophoresis with end-column amprometric detection (CE-AD) after a single-step solid phase extraction (SPE), and then quantitatively expressed as a function of creatinine excretion. To increase the concentration sensitivity, a dynamic pH junction was used and the focusing effect was obvious when using 30 mM phosphate (pH 6.50) as sample matrix. The limit of detection is 4.3 nM (signal-to-noise ratio S/N = 3). The relative standard deviation (R.S.D.) was 1.1% for peak current, and 2.3% for migration time. Based on the selected CE-AD method, it was found that the mean value of urinary 8OHdG levels in the smokers significantly higher than that in non-smokers (31.4 +/- 18.9 nM versus 14.4 +/- 7.6 nM, P = 0.0004; 23.5 +/- 21.3 mug g(-1) creatinine versus 12.6 +/- 13.2 mug g(-1) creatinine, P = 0.028). (C) 2004 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000221735500018
Citation statistics
Cited Times:37[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/81591
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Wuhan Univ, Coll Chem, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Yao, QH,Mei, SR,Weng, QF,et al. Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking[J]. TALANTA,2004,63(3):617-623.
APA Yao, QH.,Mei, SR.,Weng, QF.,Zhang, PD.,Yang, Q.,...&Xu, GW.(2004).Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking.TALANTA,63(3),617-623.
MLA Yao, QH,et al."Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking".TALANTA 63.3(2004):617-623.
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