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题名: Repeatedly usable immobilized pH gradient in a monolithic capillary column
作者: Yang, C;  Zhu, GJ;  Zhang, LH;  Zhang, WB;  Zhang, YK
关键词: immobilized pH gradient ;  isoelectric focusing ;  monolithic column
刊名: ELECTROPHORESIS
发表日期: 2004-06-01
DOI: 10.1002/elps.200405916
卷: 25, 期:12, 页:1729-1734
收录类别: SCI
文章类型: Article
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) were used to synthesize a monolithic capillary column containing reactive epoxy groups. Glutaraldehyde was introduced and linked to the monolith after a process of amination. An aqueous solution of commercial carrier ampholytes (CAs, Ampholine) was focused in such a polymer column. The primary amino groups of CAs reacted with glutaraldehyde along the capillary. CAs were immobilized at different positions in the column according to their isoelectric points (pl), resulting in a monolithic immobilized pH gradient (M-IPG). Isoelectric focusing (IEF) was performed without CAs in such an M-IPG column. Due to the covalent attachment of the CAs this M-IPG can be repeatedly used after its preparation. Good stability, linearity, and reproducibility were obtained.
关键词[WOS]: MASS-SPECTROMETRY ;  LIQUID-CHROMATOGRAPHY ;  GEL-ELECTROPHORESIS ;  2-DIMENSIONAL ELECTROPHORESIS ;  ELECTROSPRAY IONIZATION ;  MACROPOROUS POLYMER ;  CARRIER AMPHOLYTES ;  PROTEOMICS ;  SEPARATION ;  ELECTROCHROMATOGRAPHY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000222396000002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81643
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dept Biotechnol, Dalian 116011, Peoples R China

Recommended Citation:
Yang, C,Zhu, GJ,Zhang, LH,et al. Repeatedly usable immobilized pH gradient in a monolithic capillary column[J]. ELECTROPHORESIS,2004,25(12):1729-1734.
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