DICP OpenIR
Repeatedly usable immobilized pH gradient in a monolithic capillary column
Yang, C; Zhu, GJ; Zhang, LH; Zhang, WB; Zhang, YK
关键词Immobilized Ph Gradient Isoelectric Focusing Monolithic Column
刊名ELECTROPHORESIS
2004-06-01
DOI10.1002/elps.200405916
25期:12页:1729-1734
收录类别SCI
文章类型Article
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; GEL-ELECTROPHORESIS ; 2-DIMENSIONAL ELECTROPHORESIS ; ELECTROSPRAY IONIZATION ; MACROPOROUS POLYMER ; CARRIER AMPHOLYTES ; PROTEOMICS ; SEPARATION ; ELECTROCHROMATOGRAPHY
英文摘要Glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) were used to synthesize a monolithic capillary column containing reactive epoxy groups. Glutaraldehyde was introduced and linked to the monolith after a process of amination. An aqueous solution of commercial carrier ampholytes (CAs, Ampholine) was focused in such a polymer column. The primary amino groups of CAs reacted with glutaraldehyde along the capillary. CAs were immobilized at different positions in the column according to their isoelectric points (pl), resulting in a monolithic immobilized pH gradient (M-IPG). Isoelectric focusing (IEF) was performed without CAs in such an M-IPG column. Due to the covalent attachment of the CAs this M-IPG can be repeatedly used after its preparation. Good stability, linearity, and reproducibility were obtained.
语种英语
原文出处查看原文
WOS记录号WOS:000222396000002
引用统计
被引频次:31[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/81643
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dept Biotechnol, Dalian 116011, Peoples R China
推荐引用方式
GB/T 7714
Yang, C,Zhu, GJ,Zhang, LH,et al. Repeatedly usable immobilized pH gradient in a monolithic capillary column[J]. ELECTROPHORESIS,2004,25(12):1729-1734.
APA Yang, C,Zhu, GJ,Zhang, LH,Zhang, WB,&Zhang, YK.(2004).Repeatedly usable immobilized pH gradient in a monolithic capillary column.ELECTROPHORESIS,25(12),1729-1734.
MLA Yang, C,et al."Repeatedly usable immobilized pH gradient in a monolithic capillary column".ELECTROPHORESIS 25.12(2004):1729-1734.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
20049K8779TY5Y.pdf(122KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, C]的文章
[Zhu, GJ]的文章
[Zhang, LH]的文章
百度学术
百度学术中相似的文章
[Yang, C]的文章
[Zhu, GJ]的文章
[Zhang, LH]的文章
必应学术
必应学术中相似的文章
[Yang, C]的文章
[Zhu, GJ]的文章
[Zhang, LH]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。