DICP OpenIR
Repeatedly usable immobilized pH gradient in a monolithic capillary column
Yang, C; Zhu, GJ; Zhang, LH; Zhang, WB; Zhang, YK
KeywordImmobilized Ph Gradient Isoelectric Focusing Monolithic Column
Source PublicationELECTROPHORESIS
2004-06-01
DOI10.1002/elps.200405916
Volume25Issue:12Pages:1729-1734
Indexed BySCI
SubtypeArticle
Funding Project1810
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordMASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; GEL-ELECTROPHORESIS ; 2-DIMENSIONAL ELECTROPHORESIS ; ELECTROSPRAY IONIZATION ; MACROPOROUS POLYMER ; CARRIER AMPHOLYTES ; PROTEOMICS ; SEPARATION ; ELECTROCHROMATOGRAPHY
AbstractGlycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) were used to synthesize a monolithic capillary column containing reactive epoxy groups. Glutaraldehyde was introduced and linked to the monolith after a process of amination. An aqueous solution of commercial carrier ampholytes (CAs, Ampholine) was focused in such a polymer column. The primary amino groups of CAs reacted with glutaraldehyde along the capillary. CAs were immobilized at different positions in the column according to their isoelectric points (pl), resulting in a monolithic immobilized pH gradient (M-IPG). Isoelectric focusing (IEF) was performed without CAs in such an M-IPG column. Due to the covalent attachment of the CAs this M-IPG can be repeatedly used after its preparation. Good stability, linearity, and reproducibility were obtained.
Language英语
URL查看原文
WOS IDWOS:000222396000002
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/81643
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dept Biotechnol, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Yang, C,Zhu, GJ,Zhang, LH,et al. Repeatedly usable immobilized pH gradient in a monolithic capillary column[J]. ELECTROPHORESIS,2004,25(12):1729-1734.
APA Yang, C,Zhu, GJ,Zhang, LH,Zhang, WB,&Zhang, YK.(2004).Repeatedly usable immobilized pH gradient in a monolithic capillary column.ELECTROPHORESIS,25(12),1729-1734.
MLA Yang, C,et al."Repeatedly usable immobilized pH gradient in a monolithic capillary column".ELECTROPHORESIS 25.12(2004):1729-1734.
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