DICP OpenIR
Separation of acidic and basic compounds in capillary electrochromatography with polymethacrylate-based monolithic columns; Separation of acidic and basic compounds in capillary electrochromatography with polymethacrylate-based monolithic columns
Ping, GC; Zhang, LH; Zhang, L; Zhang, WB; Schmitt-Kopplin, P; Kettrup, A; Zhang, YK
KeywordElectrochromatography Electrochromatography Monolithic Columns Monolithic Columns Stationary Phases Stationary Phases Electrochromatography Electrochromatography
Source PublicationJOURNAL OF CHROMATOGRAPHY A ; JOURNAL OF CHROMATOGRAPHY A
2004-05-07 ; 2004-05-07
DOI10.1016/j.chroma.2004.02.063 ; 10.1016/j.chroma.2004.02.063
Volume1035Issue:2Pages:265-270
Indexed BySCI ; SCI
SubtypeArticle ; Article
Funding Project1810 ; 1810
Contribution Rank1;1 ; 1;1
WOS HeadingsScience & Technology ; Science & Technology ; Life Sciences & Biomedicine ; Life Sciences & Biomedicine ; Physical Sciences ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Biochemical Research Methods ; Chemistry, Analytical ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Biochemistry & Molecular Biology ; Chemistry ; Chemistry
WOS KeywordPHASE LIQUID-CHROMATOGRAPHY ; PHASE LIQUID-CHROMATOGRAPHY ; RIGID POLYMER MONOLITHS ; RIGID POLYMER MONOLITHS ; REVERSED-PHASE ; REVERSED-PHASE ; STATIONARY PHASES ; STATIONARY PHASES ; CONTINUOUS BEDS ; CONTINUOUS BEDS ; PACKED-COLUMNS ; PACKED-COLUMNS ; PERFORMANCE ; PERFORMANCE ; MEDIA ; MEDIA ; NUCLEOSIDES ; NUCLEOSIDES ; FLOW ; FLOW
AbstractMethacrylate-based monolithic columns with electroosmotic flow (EOF) or very weak EOF are prepared by in situ copolymerization in the presence of a porogen in fused-silica capillaries pretreated with a bifunctional reagent. Satisfactory separations of acidic and basic compounds on the column with EOF at either low or high pH are achieved, respectively. With sulfonic groups as dissociation functionalities, sufficient EOF mobility still remains as high as 1.74 x 10(-4) cm(2) s(-1) V-1 at low pH. Under this condition, seven acidic compounds are readily separated within 5.7 min. Moreover, at high pH, the peak shape of basic compounds is satisfactory without addition of any masking amines into running mobile phase since the secondary interaction between the basic compounds and the monolithic stationary phase are minimized at high pH. Reversed-phase mechanism for both acidic and basic compounds is observed under investigated separation conditions. In addition, possibilities of acidic and basic compound separations on a monolithic column with extremely low EOF are discussed. (C) 2004 Elsevier B.V. All rights reserved.; Methacrylate-based monolithic columns with electroosmotic flow (EOF) or very weak EOF are prepared by in situ copolymerization in the presence of a porogen in fused-silica capillaries pretreated with a bifunctional reagent. Satisfactory separations of acidic and basic compounds on the column with EOF at either low or high pH are achieved, respectively. With sulfonic groups as dissociation functionalities, sufficient EOF mobility still remains as high as 1.74 x 10(-4) cm(2) s(-1) V-1 at low pH. Under this condition, seven acidic compounds are readily separated within 5.7 min. Moreover, at high pH, the peak shape of basic compounds is satisfactory without addition of any masking amines into running mobile phase since the secondary interaction between the basic compounds and the monolithic stationary phase are minimized at high pH. Reversed-phase mechanism for both acidic and basic compounds is observed under investigated separation conditions. In addition, possibilities of acidic and basic compound separations on a monolithic column with extremely low EOF are discussed. (C) 2004 Elsevier B.V. All rights reserved.
Language英语 ; 英语
URL查看原文 ; 查看原文
WOS IDWOS:000220783100012 ; WOS:000220783100012
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/81647
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
2.GSF Natl Ctr Environm & Hlth, Inst Ecol Chem, D-85764 Neuherberg, Germany
Recommended Citation
GB/T 7714
Ping, GC,Zhang, LH,Zhang, L,et al. Separation of acidic and basic compounds in capillary electrochromatography with polymethacrylate-based monolithic columns, Separation of acidic and basic compounds in capillary electrochromatography with polymethacrylate-based monolithic columns[J]. JOURNAL OF CHROMATOGRAPHY A, JOURNAL OF CHROMATOGRAPHY A,2004, 2004,1035, 1035(2):265-270, 265-270.
APA Ping, GC.,Zhang, LH.,Zhang, L.,Zhang, WB.,Schmitt-Kopplin, P.,...&Zhang, YK.(2004).Separation of acidic and basic compounds in capillary electrochromatography with polymethacrylate-based monolithic columns.JOURNAL OF CHROMATOGRAPHY A,1035(2),265-270.
MLA Ping, GC,et al."Separation of acidic and basic compounds in capillary electrochromatography with polymethacrylate-based monolithic columns".JOURNAL OF CHROMATOGRAPHY A 1035.2(2004):265-270.
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