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Study on conical columns with different conical angles for semi-preparative liquid chromatography
Ma, JP; Chen, LX; Guan, YF
KeywordConical Columns Flow Profiles Preparative Chromatography
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2004-04-18
DOI10.1016/j.chroma.2004.01.066
Volume1033Issue:2Pages:275-281
Indexed BySCI
SubtypeArticle
Funding Project105
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordSOLUTE MIGRATION ; VISUALIZATION
AbstractThe dynamic flow profiles and separation performances in conically shaped preparative liquid chromatographic columns (inlet i.d. larger than outlet i.d.) with three different angles (7, 10 and 15degrees) were studied and compared with cylindrical column of the same length and internal volume. The shapes of dynamic flow profiles were studied by on-column visualization method. The transparent chromatographic columns made of polymethyl methacrylate (PMMA), packed with C-18 bonded silica, were immerged into a cubic pool filled with glycerol to eliminate the cylindrical and conical lens effect. The flow profiles of colored iodine solution in the columns were observed clearly using cyclohexane as mobile phase since the refractive indices of C-18, column wall and the mobile phase are very close. In the conical column of 15degrees (20-7 mm i.d.) the mobile phase in the central region migrated slower than in wall region as it moved toward the column outlet, while in the conical column of 7degrees (17-11 mm i.d.) the mobile phase in the central region migrated faster than in wall region just like in cylindrical column. We found that a plug-like flow profile was generated in the conical column of 10degrees (18-9 mm i.d.) during the whole migration process. A carmine and brilliant blue mixture was used as a probe to test the separation ability of the columns. The resolutions of the two compounds on the conical column of 7, 10, 15degrees and on the cylindrical column were 0.6, 1.57, 1.29 and 0.8, respectively. (C) 2003 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000220398900010
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/81697
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem & Microinstrumentat, Dalian 116012, Peoples R China
2.Liaoning Univ Petr & Chem Technol, Dept Appl Chem, Fushun 113001, Peoples R China
Recommended Citation
GB/T 7714
Ma, JP,Chen, LX,Guan, YF. Study on conical columns with different conical angles for semi-preparative liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2004,1033(2):275-281.
APA Ma, JP,Chen, LX,&Guan, YF.(2004).Study on conical columns with different conical angles for semi-preparative liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1033(2),275-281.
MLA Ma, JP,et al."Study on conical columns with different conical angles for semi-preparative liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1033.2(2004):275-281.
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