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题名: Study on conical columns with different conical angles for semi-preparative liquid chromatography
作者: Ma, JP;  Chen, LX;  Guan, YF
关键词: conical columns ;  flow profiles ;  preparative chromatography
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2004-04-18
DOI: 10.1016/j.chroma.2004.01.066
卷: 1033, 期:2, 页:275-281
收录类别: SCI
文章类型: Article
项目归属: 105
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The dynamic flow profiles and separation performances in conically shaped preparative liquid chromatographic columns (inlet i.d. larger than outlet i.d.) with three different angles (7, 10 and 15degrees) were studied and compared with cylindrical column of the same length and internal volume. The shapes of dynamic flow profiles were studied by on-column visualization method. The transparent chromatographic columns made of polymethyl methacrylate (PMMA), packed with C-18 bonded silica, were immerged into a cubic pool filled with glycerol to eliminate the cylindrical and conical lens effect. The flow profiles of colored iodine solution in the columns were observed clearly using cyclohexane as mobile phase since the refractive indices of C-18, column wall and the mobile phase are very close. In the conical column of 15degrees (20-7 mm i.d.) the mobile phase in the central region migrated slower than in wall region as it moved toward the column outlet, while in the conical column of 7degrees (17-11 mm i.d.) the mobile phase in the central region migrated faster than in wall region just like in cylindrical column. We found that a plug-like flow profile was generated in the conical column of 10degrees (18-9 mm i.d.) during the whole migration process. A carmine and brilliant blue mixture was used as a probe to test the separation ability of the columns. The resolutions of the two compounds on the conical column of 7, 10, 15degrees and on the cylindrical column were 0.6, 1.57, 1.29 and 0.8, respectively. (C) 2003 Elsevier B.V. All rights reserved.
关键词[WOS]: SOLUTE MIGRATION ;  VISUALIZATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000220398900010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81697
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem & Microinstrumentat, Dalian 116012, Peoples R China
2.Liaoning Univ Petr & Chem Technol, Dept Appl Chem, Fushun 113001, Peoples R China

Recommended Citation:
Ma, JP,Chen, LX,Guan, YF. Study on conical columns with different conical angles for semi-preparative liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2004,1033(2):275-281.
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