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Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application
Ye, ZQ; Tan, MQ; Wang, GL; Yuan, JL; Yuan JL(袁景利); Yuan JL(袁景利)
关键词Functionalized Terbium Nanoparticles Fluorescence Alpha-fetoprotein Time-resolved Fluoroimmunoassay
刊名TALANTA
2005-01-15
DOI10.1016/j.talanta.2004.06.008
65期:1页:206-210
收录类别SCI
文章类型Article
部门归属1
项目归属101
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PROSTATE-SPECIFIC ANTIGEN ; ALPHA-FETOPROTEIN ; ULTRASENSITIVE IMMUNOASSAYS ; IMMUNOFLUOROMETRIC ASSAY ; POLY(ETHYLENE GLYCOL) ; BIOANALYTICAL ASSAYS ; CHELATE ; SERUM ; FLUOROMETRY ; TECHNOLOGY
英文摘要Silica-based functionalized terbium fluorescent nanoparticles were prepared, characterized and developed as a fluorescence probe for antibody labeling and time-resolved fluoroimmunoassay. The nanoparticles were prepared in a water-in-oil (W/O) microemulsion containing a strongly fluorescent Tb3+ chelate. N,N.N-1,N-1-12,6-bis(3'-aminomethyl-1'-pyrazolyl)phenylpyridine] tetrakis(acetate)-Tb3+ (BPTA-Tb3+), Triton X-100, octanol, and cyclohexane by controlling copolymerization of tetraethyl orthosilicate (TEOS) and 3-[2-(2- aminoethylamino)-ethylamino]propyl-trimethoxysilane (AEPS) with ammonia water. The characterizations by transmission electron microscopy and fluorometric quantum methods show that the nanoparticles are spherical and uniform in size, 45 +/- 3 nm in diameter, strongly fluorescent with fluorescence yield of 10% and a long fluorescence lifetime of 2.0 ms. The amino groups directly introduced to the nanoparticle's surface by using AEPS in the preparation made the surface modification and bioconjugation of the nanoparticles easier. The nanoparticle-labeled anti-human alpha-fetoprotein antibody was prepared and used for time-resolved fluoroimmunoassay of (x-fetoprotein (AFP) in human serum samples. The assay response is linear from 0.10 ng ml(-1) to about 100 ng ml(-1) with the detection limit of 0.10 ng ml(-1). The coefficient variations (CVs) of the method are less than 9.0%. and the recoveries are in the range of 84-98% for human serum sample measurements. (C) 2004 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000225381600030
引用统计
被引频次:78[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/81717
专题中国科学院大连化学物理研究所
通讯作者Yuan JL(袁景利); Yuan JL(袁景利)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China
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Ye, ZQ,Tan, MQ,Wang, GL,et al. Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application[J]. TALANTA,2005,65(1):206-210.
APA Ye, ZQ,Tan, MQ,Wang, GL,Yuan, JL,袁景利,&袁景利.(2005).Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application.TALANTA,65(1),206-210.
MLA Ye, ZQ,et al."Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application".TALANTA 65.1(2005):206-210.
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