中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application
作者: Ye, ZQ;  Tan, MQ;  Wang, GL;  Yuan, JL
通讯作者: 袁景利
关键词: functionalized terbium nanoparticles ;  fluorescence ;  alpha-fetoprotein ;  time-resolved fluoroimmunoassay
刊名: TALANTA
发表日期: 2005-01-15
DOI: 10.1016/j.talanta.2004.06.008
卷: 65, 期:1, 页:206-210
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 101
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Silica-based functionalized terbium fluorescent nanoparticles were prepared, characterized and developed as a fluorescence probe for antibody labeling and time-resolved fluoroimmunoassay. The nanoparticles were prepared in a water-in-oil (W/O) microemulsion containing a strongly fluorescent Tb3+ chelate. N,N.N-1,N-1-12,6-bis(3'-aminomethyl-1'-pyrazolyl)phenylpyridine] tetrakis(acetate)-Tb3+ (BPTA-Tb3+), Triton X-100, octanol, and cyclohexane by controlling copolymerization of tetraethyl orthosilicate (TEOS) and 3-[2-(2- aminoethylamino)-ethylamino]propyl-trimethoxysilane (AEPS) with ammonia water. The characterizations by transmission electron microscopy and fluorometric quantum methods show that the nanoparticles are spherical and uniform in size, 45 +/- 3 nm in diameter, strongly fluorescent with fluorescence yield of 10% and a long fluorescence lifetime of 2.0 ms. The amino groups directly introduced to the nanoparticle's surface by using AEPS in the preparation made the surface modification and bioconjugation of the nanoparticles easier. The nanoparticle-labeled anti-human alpha-fetoprotein antibody was prepared and used for time-resolved fluoroimmunoassay of (x-fetoprotein (AFP) in human serum samples. The assay response is linear from 0.10 ng ml(-1) to about 100 ng ml(-1) with the detection limit of 0.10 ng ml(-1). The coefficient variations (CVs) of the method are less than 9.0%. and the recoveries are in the range of 84-98% for human serum sample measurements. (C) 2004 Elsevier B.V. All rights reserved.
关键词[WOS]: PROSTATE-SPECIFIC ANTIGEN ;  ALPHA-FETOPROTEIN ;  ULTRASENSITIVE IMMUNOASSAYS ;  IMMUNOFLUOROMETRIC ASSAY ;  POLY(ETHYLENE GLYCOL) ;  BIOANALYTICAL ASSAYS ;  CHELATE ;  SERUM ;  FLUOROMETRY ;  TECHNOLOGY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000225381600030
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81717
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
200551Z0E2CFP3.pdf(107KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China

Recommended Citation:
Ye, ZQ,Tan, MQ,Wang, GL,et al. Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application[J]. TALANTA,2005,65(1):206-210.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Ye, ZQ]'s Articles
 [Tan, MQ]'s Articles
 [Wang, GL]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Ye, ZQ]‘s Articles
 [Tan, MQ]‘s Articles
 [Wang, GL]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 200551Z0E2CFP3.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace