DICP OpenIR
Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application
Ye, ZQ; Tan, MQ; Wang, GL; Yuan, JL; Yuan JL(袁景利); Yuan JL(袁景利)
KeywordFunctionalized Terbium Nanoparticles Fluorescence Alpha-fetoprotein Time-resolved Fluoroimmunoassay
Source PublicationTALANTA
2005-01-15
DOI10.1016/j.talanta.2004.06.008
Volume65Issue:1Pages:206-210
Indexed BySCI
SubtypeArticle
Department1
Funding Project101
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPROSTATE-SPECIFIC ANTIGEN ; ALPHA-FETOPROTEIN ; ULTRASENSITIVE IMMUNOASSAYS ; IMMUNOFLUOROMETRIC ASSAY ; POLY(ETHYLENE GLYCOL) ; BIOANALYTICAL ASSAYS ; CHELATE ; SERUM ; FLUOROMETRY ; TECHNOLOGY
AbstractSilica-based functionalized terbium fluorescent nanoparticles were prepared, characterized and developed as a fluorescence probe for antibody labeling and time-resolved fluoroimmunoassay. The nanoparticles were prepared in a water-in-oil (W/O) microemulsion containing a strongly fluorescent Tb3+ chelate. N,N.N-1,N-1-12,6-bis(3'-aminomethyl-1'-pyrazolyl)phenylpyridine] tetrakis(acetate)-Tb3+ (BPTA-Tb3+), Triton X-100, octanol, and cyclohexane by controlling copolymerization of tetraethyl orthosilicate (TEOS) and 3-[2-(2- aminoethylamino)-ethylamino]propyl-trimethoxysilane (AEPS) with ammonia water. The characterizations by transmission electron microscopy and fluorometric quantum methods show that the nanoparticles are spherical and uniform in size, 45 +/- 3 nm in diameter, strongly fluorescent with fluorescence yield of 10% and a long fluorescence lifetime of 2.0 ms. The amino groups directly introduced to the nanoparticle's surface by using AEPS in the preparation made the surface modification and bioconjugation of the nanoparticles easier. The nanoparticle-labeled anti-human alpha-fetoprotein antibody was prepared and used for time-resolved fluoroimmunoassay of (x-fetoprotein (AFP) in human serum samples. The assay response is linear from 0.10 ng ml(-1) to about 100 ng ml(-1) with the detection limit of 0.10 ng ml(-1). The coefficient variations (CVs) of the method are less than 9.0%. and the recoveries are in the range of 84-98% for human serum sample measurements. (C) 2004 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000225381600030
Citation statistics
Cited Times:78[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/81717
Collection中国科学院大连化学物理研究所
Corresponding AuthorYuan JL(袁景利); Yuan JL(袁景利)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China
Recommended Citation
GB/T 7714
Ye, ZQ,Tan, MQ,Wang, GL,et al. Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application[J]. TALANTA,2005,65(1):206-210.
APA Ye, ZQ,Tan, MQ,Wang, GL,Yuan, JL,袁景利,&袁景利.(2005).Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application.TALANTA,65(1),206-210.
MLA Ye, ZQ,et al."Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application".TALANTA 65.1(2005):206-210.
Files in This Item:
File Name/Size DocType Version Access License
200551Z0E2CFP3.pdf(107KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ye, ZQ]'s Articles
[Tan, MQ]'s Articles
[Wang, GL]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ye, ZQ]'s Articles
[Tan, MQ]'s Articles
[Wang, GL]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ye, ZQ]'s Articles
[Tan, MQ]'s Articles
[Wang, GL]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.