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题名: Short columns with molecularly imprinted monolithic stationary phases for rapid separation of diastereomers and enantiomers
作者: Huang, XD;  Qin, F;  Chen, XM;  Liu, YQ;  Zou, HF
关键词: enantiomer separation ;  stationary phases ;  LC ;  molecularly imprinted monoliths ;  cinchonine ;  cinchonidine
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2004-05-05
DOI: 10.1016/j.jchromb.2004.01.015
卷: 804, 期:1, 页:13-18
收录类别: SCI
文章类型: Article
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Three molecularly imprinted monolithic columns with different length but almost identical column volume had been prepared. It was observed that the separation factors of diastereomers and enantiomers were almost unaffected by column length. However, the short column with dimension of 38 mm x 8 mm W. showed much lower resistance to flow rate so that it could be operated at much higher flow rates. By combining stepwise gradient elution with elevated flow rate, the diastereomers of cinchonine and cinchonidine and the enantiomers of Cbz-DL-Trp and Fmoc-DL-Trp were successfully separated within 3 min on the short column with dimension of 38 mm. x 8 mm i.d.. Based on the above results, a cinchonine imprinted monolithic disk with dimension of 10 mm x 16 mm W. was further developed. The SEM image and the pore size distribution profile showed that large flow-through pores are present on the prepared monolith, which allowed mobile phase to flow through the disk with very low resistance. Chromatographic performances on the monolithic disk were almost unchanged compared with the long columns. A rapid separation of cinchonine and cinchonidine was achieved in 2.5 min at the flow rate of 9.0 ml/min. Furthermore, it was observed that there was almost no effect of the flow rate on the dynamic binding capacity at high flow rates. In addition, the effect of the loading concentration of analytes on the dynamic binding capacity, namely adsorption isotherm, was also investigated. A non-linear adsorption isotherm of cinchonine was observed on the molecularly imprinted monolith with cinchonine as template, which might be a main reason to result in the peak tailing of template molecule. (C) 2004 Elsevier B.V. All rights reserved.
关键词[WOS]: CAPILLARY ELECTROCHROMATOGRAPHY ;  LIQUID-CHROMATOGRAPHY ;  POLYMER MONOLITHS ;  AFFINITY MEDIA ;  RECOGNITION ;  SELECTIVITY ;  BEADS ;  RODS ;  PORE ;  ACID
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000220939100003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81987
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Analysis Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Huang, XD,Qin, F,Chen, XM,et al. Short columns with molecularly imprinted monolithic stationary phases for rapid separation of diastereomers and enantiomers[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2004,804(1):13-18.
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