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Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry
Xu, SY; Pan, CS; Hu, LG; Zhang, Y; Guo, Z; Li, X; Zou, HF
KeywordDesorptiOn/iOnizatiOn On silicOn Enzyme Inhibitor Immobilized Enzyme Matrix Assisted Laser Desorption/ioniozation-time Of Flight-mass Spectrometry Miniaturization Peptide Mapping
Source PublicationELECTROPHORESIS
2004-11-01
DOI10.1002/elps.200406063
Volume25Issue:21-22Pages:3669-3676
Indexed BySCI
SubtypeArticle
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPROTEIN IDENTIFICATION ; PEPTIDE ; PROTEOMICS ; PLATFORM
AbstractDesorption/ionization on silicon mass spectrometry (DIOS-MS) is a matrix-free technique that allows for the direct desorption/ionization of low-molecular-weight compounds with little or no fragmentation of analytes. This technique has a relatively high tolerance for contaminants commonly found in biological samples. DIOS-MS has been applied to determine the activity of immobilized enzymes on the porous silicon surface. Enzyme activities were also monitored with the addition of a competitive inhibitor in the substrate solution. It is demonstrated that this method can be applied to the screening of enzyme inhibitors. Furthermore, a method for peptide mapping analysis by in situ digestion of proteins on the porous silicon surface modified by trypsin, combined with matrix-assisted laser desorption/ionization-time of flight-MS has been developed.
Language英语
URL查看原文
WOS IDWOS:000225679000016
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/81995
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Xu, SY,Pan, CS,Hu, LG,et al. Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry[J]. ELECTROPHORESIS,2004,25(21-22):3669-3676.
APA Xu, SY.,Pan, CS.,Hu, LG.,Zhang, Y.,Guo, Z.,...&Zou, HF.(2004).Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry.ELECTROPHORESIS,25(21-22),3669-3676.
MLA Xu, SY,et al."Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry".ELECTROPHORESIS 25.21-22(2004):3669-3676.
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