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题名: Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry
作者: Xu, SY;  Pan, CS;  Hu, LG;  Zhang, Y;  Guo, Z;  Li, X;  Zou, HF
关键词: desorption/ionization on silicon ;  enzyme inhibitor ;  immobilized enzyme ;  matrix assisted laser desorption/ioniozation-time of flight-mass spectrometry ;  miniaturization ;  peptide mapping
刊名: ELECTROPHORESIS
发表日期: 2004-11-01
DOI: 10.1002/elps.200406063
卷: 25, 期:21-22, 页:3669-3676
收录类别: SCI
文章类型: Article
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Desorption/ionization on silicon mass spectrometry (DIOS-MS) is a matrix-free technique that allows for the direct desorption/ionization of low-molecular-weight compounds with little or no fragmentation of analytes. This technique has a relatively high tolerance for contaminants commonly found in biological samples. DIOS-MS has been applied to determine the activity of immobilized enzymes on the porous silicon surface. Enzyme activities were also monitored with the addition of a competitive inhibitor in the substrate solution. It is demonstrated that this method can be applied to the screening of enzyme inhibitors. Furthermore, a method for peptide mapping analysis by in situ digestion of proteins on the porous silicon surface modified by trypsin, combined with matrix-assisted laser desorption/ionization-time of flight-MS has been developed.
关键词[WOS]: PROTEIN IDENTIFICATION ;  PEPTIDE ;  PROTEOMICS ;  PLATFORM
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000225679000016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81995
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, Beijing, Peoples R China

Recommended Citation:
Xu, SY,Pan, CS,Hu, LG,et al. Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry[J]. ELECTROPHORESIS,2004,25(21-22):3669-3676.
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