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Study on conical columns for semi-preparative liquid chromatography
Ma, JP; Guan, YF; Chen, LX
KeywordConical Column Flow Profile Zone Shape Semi-preparative Liquid Chromatography
Source PublicationJOURNAL OF SEPARATION SCIENCE
2003-03-01
Volume26Issue:3-4Pages:307-312
Indexed BySCI
SubtypeArticle
Department105
Funding Project105
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordSOLUTE MIGRATION ; VISUALIZATION ; DIAMETER
AbstractThe dynamic flow profiles and column efficiencies in conically shaped semi-preparative liquid chromatographic columns (inlet ID larger than outlet ID) with two different conical angles (7degrees and 15degrees) were studied. The dynamic flow profiles were studied by an on-column visualization method. Conical columns were compared with cylindrical column of the same length and internal volume. The results showed that the flow profile of a sample band in the conical column of 7degrees (50 mm x 17 mm --> 11 mm ID) was parabolic in shape. The sample band migrated slower in the wall region than in the central region, as in the cylindrical column (50 mm x 14 mm ID). However, the sample band in the conical column of 15degrees (50 mm x 20 mm --> 7 mm ID) migrated slower in the central region than in the wall region, resulting in a reverse parabolic flow profile, in contrast to that in cylindrical column. This indicated that a flat flow profile might be realized in a conical column with a conical angle between 7degrees and 15degrees. The conical column of 15degrees had the highest column efficiency among the three columns under the same conditions. Compared with the cylindrical column packed with identical packing material, the conical column of 15degrees had 22%-45% higher column efficiency and 11%-27% higher peak height.
Language英语
URL查看原文
WOS IDWOS:000181950200014
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/82153
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem & Micro Instrumentat, Dalian 116012, Peoples R China
2.Liaoning Univ Petr & Chem Technol, Dept Appl Chem, Fushun 113001, Peoples R China
Recommended Citation
GB/T 7714
Ma, JP,Guan, YF,Chen, LX. Study on conical columns for semi-preparative liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2003,26(3-4):307-312.
APA Ma, JP,Guan, YF,&Chen, LX.(2003).Study on conical columns for semi-preparative liquid chromatography.JOURNAL OF SEPARATION SCIENCE,26(3-4),307-312.
MLA Ma, JP,et al."Study on conical columns for semi-preparative liquid chromatography".JOURNAL OF SEPARATION SCIENCE 26.3-4(2003):307-312.
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