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题名: Unilamellar liposomes covalently coupled on silica gel for liquid chromatography
作者: Mao, XQ;  Kong, L;  Li, X;  Guo, BC;  Zou, HF
关键词: covalent coupling ;  liposome ;  silica gel ;  peptides ;  Chinese medicine
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2003-02-01
DOI: 10.1007/s00216-002-1721-0
卷: 375, 期:4, 页:550-555
收录类别: SCI
文章类型: Article
部门归属: 1809
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Silica gel was used as a support for the covalent coupling of liposomes, which could overcome drawbacks of soft gel beads in column efficiency and separation speed. The influences of the concentration of added dimethylaminopyridine and reaction time on the chloroformate activation reaction of silica gel were investigated. Temperature and pH for covalent coupling of liposomes on the activated silica gel were also optimized. Experimental results indicated that the stability of the covalently coupled liposome columns was obviously superior to that of the noncovalently coated liposome columns but the selectivity of both columns was basically identical. Separation and analysis of a crude extract of a traditional Chinese medicine Ligusticum Wallichii and a mixture of small peptides on both columns further support this conclusion.
关键词[WOS]: IMMOBILIZED-LIPOSOME ;  BIOMEMBRANE CHROMATOGRAPHY ;  MEMBRANE INTERACTIONS ;  DRUGS ;  PROTEINS ;  BEADS ;  PREDICTION ;  COLUMN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000181659600014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/82391
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Mao, XQ,Kong, L,Li, X,et al. Unilamellar liposomes covalently coupled on silica gel for liquid chromatography[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2003,375(4):550-555.
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