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题名: Analysis of specific gene by integration of isothermal amplification and electrophoresis on poly(methyl methacrylate) microchips
作者: Hataoka, Y;  Zhang, LH;  Mori, Y;  Tomita, N;  Notomi, T;  Baba, Y
通讯作者: 张丽华
刊名: ANALYTICAL CHEMISTRY
发表日期: 2004-07-01
DOI: 10.1021/ac035032u
卷: 76, 期:13, 页:3689-3693
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: We have successfully achieved the integration of isothermal amplification and the subsequent analysis of specific gene fragments on poly(methyl methacrylate) microchips. In our experiments, loop-mediated isothermal amplification, which can offer higher specificity and efficiency than PCR, has been performed at a constant temperature (65 degreesC). After amplification, products could be either examined by the integrated microchip-based electrophoresis or directly observed by naked eye with SYBR Green I added into the reaction solution. By such an integrated microsystem, the amplification and the subsequent analysis of prostate-specific antigen gene with template concentration at 23 fg/muL could be finished within 15 min, which demonstrates its advantages of high specificity, good reproducibility, and fast speed in gene detection.
关键词[WOS]: DNA AMPLIFICATION ;  DEVICE ;  SEPARATION ;  SYSTEMS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000222418300037
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/82565
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Tokushima, Grad Sch Pharmaceut Sci, Dept Mol & Phamaceut Biotechnol, CREST,JST, Tokushima 7708505, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
3.Natl Inst Adv Ind Sci & Technol, AIST, Single Mol Bioanal Lab, Takamatsu, Kagawa 7610395, Japan

Recommended Citation:
Hataoka, Y,Zhang, LH,Mori, Y,et al. Analysis of specific gene by integration of isothermal amplification and electrophoresis on poly(methyl methacrylate) microchips[J]. ANALYTICAL CHEMISTRY,2004,76(13):3689-3693.
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