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题名: Membrane cartridges for endotoxin removal from interferon preparations
作者: Li, JH;  Shao, YG;  Chen, ZA;  Cong, RZ;  Wang, JD;  Liu, XL
关键词: membrane cartridges ;  cellulose membrane ;  interferon ;  chitosan ;  deoxycholate ;  endotoxin
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2003-07-05
DOI: 10.1016/S1570-0232(03)00203-4
卷: 791, 期:1-2, 页:55-61
收录类别: SCI
文章类型: Article
部门归属: 102
项目归属: 102
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The suitability of membrane cartridges for the removal of endotoxin from both distilled water and interferon preparations was examined. The endotoxin concentrations were reduced to 4.0 and 7.3 EU/ml, respectively, when about 4000 ml of distilled water with 20 and 28 EU/ml were passed through the deoxycholate and chitosan immobilized membrane cartridges. When 200 ml of interferon preparation with endotoxin concentration more than 80 EU/ml and pH 3.9 were applied to a deoxycholate immobilized membrane cartridge at a flow-rate of 9 ml/min, the endotoxin concentration was reduced to less than 10 EU/ml. However, if an interferon preparation of 450 ml, with more than 80 EU/ml of endotoxin and pH 3.9 was applied to the chitosan immobilized membrane cartridge at a flow-rate of 18 ml/min, the endotoxin concentration was reduced to less than 10 EU/ml. (C) 2003 Elsevier Science B.V. All rights reserved.
关键词[WOS]: AFFINITY SORBENTS ;  ADSORBERS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000183646000007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/82725
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China

Recommended Citation:
Li, JH,Shao, YG,Chen, ZA,et al. Membrane cartridges for endotoxin removal from interferon preparations[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2003,791(1-2):55-61.
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