DICP OpenIR
Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers
Zhao, QY; Jin, MF; Muller, WEG; Zhang, W; Yu, XJ; Deng, MC
Source PublicationBIOTECHNOLOGY PROGRESS
2003-09-01
DOI10.1021/bp034106k
Volume19Issue:5Pages:1569-1573
Indexed BySCI
SubtypeArticle
Department1812
Funding Project1812
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology ; Food Science & Technology
WOS Research AreaBiotechnology & Applied Microbiology ; Food Science & Technology
WOS KeywordSUBERITES-DOMUNCULA ; VERO CELLS ; DYSIDEA-AVARA ; PRIMMORPHS ; CULTURE ; CULTIVATION ; GROWTH ; PROTEINS ; KINETICS ; ORIGIN
AbstractToward the development of an in vitro cultivation of marine sponge cells for sustainable production of bioactive metabolites, the attachment characteristics of marine sponge cells of Hymeniacidon perleve on three types of microcarriers, Hillex, Cytodex 3, and glass beads, were studied. Mixed cell population and enriched cell fractions of specific cell types by Ficoll gradient centrifugation (6%/8%/15%/20%) were also assessed. Cell attachment ratio (defined as the ratio of cells attached on microcarrier to the total number of cells in the culture) on glass beads is much higher than that on Cytodex 3 and Hillex for both mixed cell population and cell fraction at Ficoll 15-20% interface. The highest attachment ratio of 41% was obtained for the cell fraction at Ficoll 15-20% interface on glass beads, which was significantly higher than that of a mixed cell population (18%). The attachment kinetics on glass beads indicated that the attachment was completed within 1 h. Cell attachment ratio decreases with increase in cell-to-microcarrier ratio (3-30 cells/bead) and pH (7.6-9.0). The addition of serum and BSA (bovine serum albumin) reduced the cell attachment on glass beads.
Language英语
URL查看原文
WOS IDWOS:000185816400024
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/82757
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Univ Mainz, Inst Physiol Chem, Angew Mol Biol Abt, D-55099 Mainz, Germany
3.Flinders Univ S Australia, Sch Med, CRC Bioprod, Bedford Pk, SA 5042, Australia
4.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia
Recommended Citation
GB/T 7714
Zhao, QY,Jin, MF,Muller, WEG,et al. Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers[J]. BIOTECHNOLOGY PROGRESS,2003,19(5):1569-1573.
APA Zhao, QY,Jin, MF,Muller, WEG,Zhang, W,Yu, XJ,&Deng, MC.(2003).Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers.BIOTECHNOLOGY PROGRESS,19(5),1569-1573.
MLA Zhao, QY,et al."Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers".BIOTECHNOLOGY PROGRESS 19.5(2003):1569-1573.
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