中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers
作者: Zhao, QY;  Jin, MF;  Muller, WEG;  Zhang, W;  Yu, XJ;  Deng, MC
刊名: BIOTECHNOLOGY PROGRESS
发表日期: 2003-09-01
DOI: 10.1021/bp034106k
卷: 19, 期:5, 页:1569-1573
收录类别: SCI
文章类型: Article
部门归属: 1812
项目归属: 1812
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ;  Food Science & Technology
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Food Science & Technology
英文摘要: Toward the development of an in vitro cultivation of marine sponge cells for sustainable production of bioactive metabolites, the attachment characteristics of marine sponge cells of Hymeniacidon perleve on three types of microcarriers, Hillex, Cytodex 3, and glass beads, were studied. Mixed cell population and enriched cell fractions of specific cell types by Ficoll gradient centrifugation (6%/8%/15%/20%) were also assessed. Cell attachment ratio (defined as the ratio of cells attached on microcarrier to the total number of cells in the culture) on glass beads is much higher than that on Cytodex 3 and Hillex for both mixed cell population and cell fraction at Ficoll 15-20% interface. The highest attachment ratio of 41% was obtained for the cell fraction at Ficoll 15-20% interface on glass beads, which was significantly higher than that of a mixed cell population (18%). The attachment kinetics on glass beads indicated that the attachment was completed within 1 h. Cell attachment ratio decreases with increase in cell-to-microcarrier ratio (3-30 cells/bead) and pH (7.6-9.0). The addition of serum and BSA (bovine serum albumin) reduced the cell attachment on glass beads.
关键词[WOS]: SUBERITES-DOMUNCULA ;  VERO CELLS ;  DYSIDEA-AVARA ;  PRIMMORPHS ;  CULTURE ;  CULTIVATION ;  GROWTH ;  PROTEINS ;  KINETICS ;  ORIGIN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000185816400024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/82757
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
20038414S681LV.pdf(582KB)----开放获取--View 联系获取全文

作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Univ Mainz, Inst Physiol Chem, Angew Mol Biol Abt, D-55099 Mainz, Germany
3.Flinders Univ S Australia, Sch Med, CRC Bioprod, Bedford Pk, SA 5042, Australia
4.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia

Recommended Citation:
Zhao, QY,Jin, MF,Muller, WEG,et al. Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers[J]. BIOTECHNOLOGY PROGRESS,2003,19(5):1569-1573.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhao, QY]'s Articles
 [Jin, MF]'s Articles
 [Muller, WEG]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhao, QY]‘s Articles
 [Jin, MF]‘s Articles
 [Muller, WEG]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 20038414S681LV.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace