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Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers
Zhao, QY; Jin, MF; Muller, WEG; Zhang, W; Yu, XJ; Deng, MC
刊名BIOTECHNOLOGY PROGRESS
2003-09-01
DOI10.1021/bp034106k
19期:5页:1569-1573
收录类别SCI
文章类型Article
部门归属1812
项目归属1812
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology ; Food Science & Technology
研究领域[WOS]Biotechnology & Applied Microbiology ; Food Science & Technology
关键词[WOS]SUBERITES-DOMUNCULA ; VERO CELLS ; DYSIDEA-AVARA ; PRIMMORPHS ; CULTURE ; CULTIVATION ; GROWTH ; PROTEINS ; KINETICS ; ORIGIN
英文摘要Toward the development of an in vitro cultivation of marine sponge cells for sustainable production of bioactive metabolites, the attachment characteristics of marine sponge cells of Hymeniacidon perleve on three types of microcarriers, Hillex, Cytodex 3, and glass beads, were studied. Mixed cell population and enriched cell fractions of specific cell types by Ficoll gradient centrifugation (6%/8%/15%/20%) were also assessed. Cell attachment ratio (defined as the ratio of cells attached on microcarrier to the total number of cells in the culture) on glass beads is much higher than that on Cytodex 3 and Hillex for both mixed cell population and cell fraction at Ficoll 15-20% interface. The highest attachment ratio of 41% was obtained for the cell fraction at Ficoll 15-20% interface on glass beads, which was significantly higher than that of a mixed cell population (18%). The attachment kinetics on glass beads indicated that the attachment was completed within 1 h. Cell attachment ratio decreases with increase in cell-to-microcarrier ratio (3-30 cells/bead) and pH (7.6-9.0). The addition of serum and BSA (bovine serum albumin) reduced the cell attachment on glass beads.
语种英语
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WOS记录号WOS:000185816400024
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/82757
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Univ Mainz, Inst Physiol Chem, Angew Mol Biol Abt, D-55099 Mainz, Germany
3.Flinders Univ S Australia, Sch Med, CRC Bioprod, Bedford Pk, SA 5042, Australia
4.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia
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GB/T 7714
Zhao, QY,Jin, MF,Muller, WEG,et al. Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers[J]. BIOTECHNOLOGY PROGRESS,2003,19(5):1569-1573.
APA Zhao, QY,Jin, MF,Muller, WEG,Zhang, W,Yu, XJ,&Deng, MC.(2003).Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers.BIOTECHNOLOGY PROGRESS,19(5),1569-1573.
MLA Zhao, QY,et al."Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers".BIOTECHNOLOGY PROGRESS 19.5(2003):1569-1573.
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