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题名: Primmorphs from archaeocytes-dominant cell population of the sponge Hymeniacidon perleve: Improved cell proliferation and spiculogenesis
作者: Zhang, XY;  Cao, XP;  Zhang, W;  Yu, XJ;  Jin, MF
关键词: porifera ;  sponges ;  primmorph ;  archaeocytes ;  cell culture ;  spiculogenesis ;  Hymeniacidon perleve
刊名: BIOTECHNOLOGY AND BIOENGINEERING
发表日期: 2003-12-05
DOI: 10.1002/bit.10811
卷: 84, 期:5, 页:583-590
收录类别: SCI
文章类型: Article
部门归属: 1812
项目归属: 1812
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Marine sponges (Porifera) possess an extraordinary diversity of bioactive metabolites for new drug discovery and development. In vitro cultivation of sponge cells in a bioreactor system is very attractive for the sustainable production of sponge-derived bioactive metabolites; however, it is still a challenging task. The recent establishment of sponge primmorphs, multicellular aggregates from dissociated mixed-cell population (MCP), has been widely acknowledged to hold great promise for cultivation in vitro. Here we present a new method to establish an in vitro sponge primmorph culture from archaeocyte-dominant cell population (ADCP) enriched by a Ficoll gradient, rather than a mixed-cell population (MCP). Our rationale is based upon the totipotency (the ability of a cell to differentiate into other cell types) of archaeocyte cells and the different biological functions of various sponge cell types. A sponge, Hymeniacidon perleve collected from the China Yellow Sea was used as a model system for this investigation. Distinct dynamics of primmorph formation were observed while significant increases in DNA synthesis, cell proliferation (up to threefold), and cell growth (up to fourfold) were achieved. Furthermore, a time-dependent spiculogenesis was clearly demonstrated in our longterm culture, indicating high metabolic activity of primmorphs from the ADCP. This new method represents an important step forward to advance sponge cell culture in vitro that may lead to commercial exploitation of sponge-derived drugs. (C) 2003 Wiley Periodicals, Inc.
关键词[WOS]: MARINE NATURAL-PRODUCTS ;  SUBERITES-DOMUNCULA ;  DYSIDEA-AVARA ;  IN-VITRO ;  FRACTIONATION ;  CULTIVATION ;  CULTURE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000186316800008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/82781
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Flinders Univ S Australia, CRC Bioprod, Bedford Pk, SA 5042, Australia
3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia

Recommended Citation:
Zhang, XY,Cao, XP,Zhang, W,et al. Primmorphs from archaeocytes-dominant cell population of the sponge Hymeniacidon perleve: Improved cell proliferation and spiculogenesis[J]. BIOTECHNOLOGY AND BIOENGINEERING,2003,84(5):583-590.
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