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Primmorphs from archaeocytes-dominant cell population of the sponge Hymeniacidon perleve: Improved cell proliferation and spiculogenesis
Zhang, XY; Cao, XP; Zhang, W; Yu, XJ; Jin, MF
关键词Porifera Sponges Primmorph Archaeocytes Cell Culture Spiculogenesis Hymeniacidon Perleve
刊名BIOTECHNOLOGY AND BIOENGINEERING
2003-12-05
DOI10.1002/bit.10811
84期:5页:583-590
收录类别SCI
文章类型Article
部门归属1812
项目归属1812
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]MARINE NATURAL-PRODUCTS ; SUBERITES-DOMUNCULA ; DYSIDEA-AVARA ; IN-VITRO ; FRACTIONATION ; CULTIVATION ; CULTURE
英文摘要Marine sponges (Porifera) possess an extraordinary diversity of bioactive metabolites for new drug discovery and development. In vitro cultivation of sponge cells in a bioreactor system is very attractive for the sustainable production of sponge-derived bioactive metabolites; however, it is still a challenging task. The recent establishment of sponge primmorphs, multicellular aggregates from dissociated mixed-cell population (MCP), has been widely acknowledged to hold great promise for cultivation in vitro. Here we present a new method to establish an in vitro sponge primmorph culture from archaeocyte-dominant cell population (ADCP) enriched by a Ficoll gradient, rather than a mixed-cell population (MCP). Our rationale is based upon the totipotency (the ability of a cell to differentiate into other cell types) of archaeocyte cells and the different biological functions of various sponge cell types. A sponge, Hymeniacidon perleve collected from the China Yellow Sea was used as a model system for this investigation. Distinct dynamics of primmorph formation were observed while significant increases in DNA synthesis, cell proliferation (up to threefold), and cell growth (up to fourfold) were achieved. Furthermore, a time-dependent spiculogenesis was clearly demonstrated in our longterm culture, indicating high metabolic activity of primmorphs from the ADCP. This new method represents an important step forward to advance sponge cell culture in vitro that may lead to commercial exploitation of sponge-derived drugs. (C) 2003 Wiley Periodicals, Inc.
语种英语
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WOS记录号WOS:000186316800008
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被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/82781
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Flinders Univ S Australia, CRC Bioprod, Bedford Pk, SA 5042, Australia
3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia
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Zhang, XY,Cao, XP,Zhang, W,et al. Primmorphs from archaeocytes-dominant cell population of the sponge Hymeniacidon perleve: Improved cell proliferation and spiculogenesis[J]. BIOTECHNOLOGY AND BIOENGINEERING,2003,84(5):583-590.
APA Zhang, XY,Cao, XP,Zhang, W,Yu, XJ,&Jin, MF.(2003).Primmorphs from archaeocytes-dominant cell population of the sponge Hymeniacidon perleve: Improved cell proliferation and spiculogenesis.BIOTECHNOLOGY AND BIOENGINEERING,84(5),583-590.
MLA Zhang, XY,et al."Primmorphs from archaeocytes-dominant cell population of the sponge Hymeniacidon perleve: Improved cell proliferation and spiculogenesis".BIOTECHNOLOGY AND BIOENGINEERING 84.5(2003):583-590.
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