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Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley)
Zhang, W; Zhang, XY; Cao, XP; Xu, JY; Zhao, QY; Yu, XJ; Jin, MF; Deng, MC
KeywordSponges Primmorph In Vitro Cultivation Stylotella Agminata Optimization
Source PublicationJOURNAL OF BIOTECHNOLOGY
2003-01-23
Volume100Issue:2Pages:161-168
Indexed ByISTP ; SCI
SubtypeArticle
Department1812
Funding Project1812
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology
WOS Research AreaBiotechnology & Applied Microbiology
WOS KeywordPRIMARY-CELL CULTURE ; SUBERITES-DOMUNCULA
AbstractThe establishment and optimization of in vitro primmorph formation from a Chinese sponge, Stylotella agminata (Ridley), collected from the South China Sea, were investigated. Our aims were to identify the key factors affecting primmorph formation in this species and to optimize the technique for developing an in vitro primmorph culture system. The size of dissociated cells from S. agminata is relatively small, in the range between 5 and 10 mum. Round-shaped primmorphs of less than 100 gm were formed 3 days after transferring the dissociated cells into seawater containing Ca2+ and Mg2+. The effect of various cell dissociation conditions, inoculum. cell density, concentration of antibiotics, pH, and temperature was further investigated upon the formation of primmorphs. The time required for primmorph formation, primmorph size distribution, and the proliferating capability were microscopically documented. Healthy sponge S. agminata, inoculum. cell density and culture temperature play a critical role for the successful formation of primmorphs and that the microbial contamination will have to be controlled. (C) 2002 Elsevier Science B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000180446200008
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/82783
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhang, W,Zhang, XY,Cao, XP,et al. Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley)[J]. JOURNAL OF BIOTECHNOLOGY,2003,100(2):161-168.
APA Zhang, W.,Zhang, XY.,Cao, XP.,Xu, JY.,Zhao, QY.,...&Deng, MC.(2003).Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley).JOURNAL OF BIOTECHNOLOGY,100(2),161-168.
MLA Zhang, W,et al."Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley)".JOURNAL OF BIOTECHNOLOGY 100.2(2003):161-168.
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