DICP OpenIR
Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley)
Zhang, W; Zhang, XY; Cao, XP; Xu, JY; Zhao, QY; Yu, XJ; Jin, MF; Deng, MC
关键词Sponges Primmorph In Vitro Cultivation Stylotella Agminata Optimization
刊名JOURNAL OF BIOTECHNOLOGY
2003-01-23
100期:2页:161-168
收录类别ISTP ; SCI
文章类型Article
部门归属1812
项目归属1812
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]PRIMARY-CELL CULTURE ; SUBERITES-DOMUNCULA
英文摘要The establishment and optimization of in vitro primmorph formation from a Chinese sponge, Stylotella agminata (Ridley), collected from the South China Sea, were investigated. Our aims were to identify the key factors affecting primmorph formation in this species and to optimize the technique for developing an in vitro primmorph culture system. The size of dissociated cells from S. agminata is relatively small, in the range between 5 and 10 mum. Round-shaped primmorphs of less than 100 gm were formed 3 days after transferring the dissociated cells into seawater containing Ca2+ and Mg2+. The effect of various cell dissociation conditions, inoculum. cell density, concentration of antibiotics, pH, and temperature was further investigated upon the formation of primmorphs. The time required for primmorph formation, primmorph size distribution, and the proliferating capability were microscopically documented. Healthy sponge S. agminata, inoculum. cell density and culture temperature play a critical role for the successful formation of primmorphs and that the microbial contamination will have to be controlled. (C) 2002 Elsevier Science B.V. All rights reserved.
语种英语
原文出处查看原文
WOS记录号WOS:000180446200008
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/82783
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Zhang, W,Zhang, XY,Cao, XP,et al. Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley)[J]. JOURNAL OF BIOTECHNOLOGY,2003,100(2):161-168.
APA Zhang, W.,Zhang, XY.,Cao, XP.,Xu, JY.,Zhao, QY.,...&Deng, MC.(2003).Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley).JOURNAL OF BIOTECHNOLOGY,100(2),161-168.
MLA Zhang, W,et al."Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley)".JOURNAL OF BIOTECHNOLOGY 100.2(2003):161-168.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
20035H6VG0994Z.pdf(244KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, W]的文章
[Zhang, XY]的文章
[Cao, XP]的文章
百度学术
百度学术中相似的文章
[Zhang, W]的文章
[Zhang, XY]的文章
[Cao, XP]的文章
必应学术
必应学术中相似的文章
[Zhang, W]的文章
[Zhang, XY]的文章
[Cao, XP]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。