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题名: Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley)
作者: Zhang, W;  Zhang, XY;  Cao, XP;  Xu, JY;  Zhao, QY;  Yu, XJ;  Jin, MF;  Deng, MC
关键词: sponges ;  primmorph ;  in vitro cultivation ;  Stylotella agminata ;  optimization
刊名: JOURNAL OF BIOTECHNOLOGY
发表日期: 2003-01-23
卷: 100, 期:2, 页:161-168
收录类别: ISTP ;  SCI
文章类型: Article
部门归属: 1812
项目归属: 1812
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: The establishment and optimization of in vitro primmorph formation from a Chinese sponge, Stylotella agminata (Ridley), collected from the South China Sea, were investigated. Our aims were to identify the key factors affecting primmorph formation in this species and to optimize the technique for developing an in vitro primmorph culture system. The size of dissociated cells from S. agminata is relatively small, in the range between 5 and 10 mum. Round-shaped primmorphs of less than 100 gm were formed 3 days after transferring the dissociated cells into seawater containing Ca2+ and Mg2+. The effect of various cell dissociation conditions, inoculum. cell density, concentration of antibiotics, pH, and temperature was further investigated upon the formation of primmorphs. The time required for primmorph formation, primmorph size distribution, and the proliferating capability were microscopically documented. Healthy sponge S. agminata, inoculum. cell density and culture temperature play a critical role for the successful formation of primmorphs and that the microbial contamination will have to be controlled. (C) 2002 Elsevier Science B.V. All rights reserved.
关键词[WOS]: PRIMARY-CELL CULTURE ;  SUBERITES-DOMUNCULA
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000180446200008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/82783
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, W,Zhang, XY,Cao, XP,et al. Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley)[J]. JOURNAL OF BIOTECHNOLOGY,2003,100(2):161-168.
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