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题名: The shape parameter of Liposomes and DNA-lipid complexes determined by viscometry utilizing small sample volumes
作者: Sun, Y;  Li, X;  Duzgunes, N;  Takaoka, Y;  Ohi, S;  Hirota, S
刊名: BIOPHYSICAL JOURNAL
发表日期: 2003-08-01
卷: 85, 期:2, 页:1223-1232
收录类别: SCI
文章类型: Article
部门归属: 1816
项目归属: 1816
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biophysics
研究领域[WOS]: Biophysics
英文摘要: A minicapillary viscometer utilizing <0.5 ml of sample at a volume fraction of <0.1% is described. The calculated a/b of DPPC/DPPG multilamellar liposome was 1.14 as prolate ellipsoids and a/b of dioleoylpropyltrimethyl ammonium methylsulfate-DNA complex at a charge ratio of 4: 1 (+/-) was 3.7 as prolate ellipsoids or 4.9 as oblate ellipsoids. The deviation of shape from perfect sphere is thus expressed quantitatively in more than two significant figures. In these measurement, the necessary amount of DNA is <0.5 mg.
关键词[WOS]: MEDIATED GENE-TRANSFER ;  TRANSFECTION ;  DELIVERY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000184428300048
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/82785
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.Univ Pacific, Sch Dent, Dept Microbiol, San Francisco, CA 94115 USA
3.Tokyo Denki Univ, Sch Engn, Tokyo 101, Japan

Recommended Citation:
Sun, Y,Li, X,Duzgunes, N,et al. The shape parameter of Liposomes and DNA-lipid complexes determined by viscometry utilizing small sample volumes[J]. BIOPHYSICAL JOURNAL,2003,85(2):1223-1232.
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