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题名: A single-strand conformation polymorphism method by capillary electrophoresis with laser-induced fluorescence for detection of the T1151A mutation in hMLH1 gene
作者: Shi, XZ;  Xu, GW;  Zhao, CX;  Ma, JM;  Zhang, Y;  Lv, S;  Yang, Q
关键词: colorectal cancer hMLH1 gene ;  lung cancer ;  single-strand conformation polymorphism
刊名: ELECTROPHORESIS
发表日期: 2003-07-01
DOI: 10.1002/elps.200305444
卷: 24, 期:14, 页:2316-2321
收录类别: SCI
文章类型: Article
部门归属: 1808
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Mutation of hMLH1 gene plays an important role in human tumorigenesis. A highly sensitive single-strand conformation polymorphism (SSCP) method for detection of the T1151A mutation in exon 12 of the hMLH1 gene was for the first time developed employing laser-induced fluorescence capillary electrophoresis (LIF-CE). Effects of the concentration of linear polyacrylamide solution, running temperature, running voltage and the addition of glycerol on SSCP analysis were investigated, and the optimum separation conditions were defined. Thirty colorectal cancer patients and eight lung cancer patients were screened and the T1151A mutation was found in four of them. Based on CE-sequencing the mutation was further confirmed. To our knowledge, this is for the first time that the T1151A mutation is found in lung cancer. Our method is simple, rapid, and highly sensitive and is well suited to the analysis of large numbers of clinical samples.
关键词[WOS]: POINT MUTATIONS ;  DNA FRAGMENTS ;  MISMATCH REPAIR ;  HIGH-THROUGHPUT ;  SIEVING MEDIUM ;  RECEPTOR GENE ;  SEPARATION ;  EFFICIENT ;  ALLELE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000184525200008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/83121
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian, Peoples R China

Recommended Citation:
Shi, XZ,Xu, GW,Zhao, CX,et al. A single-strand conformation polymorphism method by capillary electrophoresis with laser-induced fluorescence for detection of the T1151A mutation in hMLH1 gene[J]. ELECTROPHORESIS,2003,24(14):2316-2321.
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