中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Simultaneous genotyping of multiplex single nucleotide polymorphisms of the K-ras gene with a home-made kit
作者: Zhao, CX;  Xu, GW;  Shi, XZ;  Ma, JM;  Zhang, Y;  Lv, S;  Yang, Q
关键词: K-ras gene ;  single nucleotide polymorphism ;  SNaPshot
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2003-09-25
DOI: 10.1016/S1570-0232(03)00493-8
卷: 795, 期:1, 页:55-60
收录类别: SCI
文章类型: Article
部门归属: 1808
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Accurate and fast genotyping of single nucleotide polymorphisms (SNPs) is important in the human genome project. Here an automated fluorescent method that can rapidly and accurately genotype multiplex known SNPs was developed by using a homemade kit, which has lower cost but higher resolution than commercial kit. With this method, oncogene K-ras was investigated, four known SNPs of K-ras gene exon 1 in 31 coloerctal cancer patients were detected. Results indicate that mutations were present in 8(26%) of 31 patients, and most mutations were localized in codon 12. The presence of these mutations is thought to be a critical step and plays an important role in human colorectal carcinogenesisas. (C) 2003 Elsevier B.V. All rights reserved.
关键词[WOS]: STRAND CONFORMATION POLYMORPHISM ;  LINEAR POLYACRYLAMIDE SOLUTIONS ;  SEQUENCING EXTENSION PRODUCTS ;  CAPILLARY-ELECTROPHORESIS ;  COLORECTAL-CANCER ;  ONCOGENES ;  MUTATIONS ;  P53 ;  SEPARATION ;  BASES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000185270400007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/83127
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
200372J3156P8P.pdf(122KB)----开放获取--View 联系获取全文

作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China

Recommended Citation:
Zhao, CX,Xu, GW,Shi, XZ,et al. Simultaneous genotyping of multiplex single nucleotide polymorphisms of the K-ras gene with a home-made kit[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2003,795(1):55-60.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhao, CX]'s Articles
 [Xu, GW]'s Articles
 [Shi, XZ]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhao, CX]‘s Articles
 [Xu, GW]‘s Articles
 [Shi, XZ]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 200372J3156P8P.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace