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题名: Separation of peptides on mixed mode of reversed-phase and ion-exchange capillary electrochromatography with a monolithic column
作者: Wu, RA;  Zou, HF;  Fu, HJ;  Jin, WH;  Ye, ML
关键词: capillary electrochromatography ;  monolithic column ;  peptides ;  reserved phase ;  strong cation exchange
刊名: ELECTROPHORESIS
发表日期: 2002-05-01
卷: 23, 期:9, 页:1239-1245
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The mixed mode of reversed phase (RP) and strong canon-exchange (SCX) capillary electrochromatography (CEC) based on a monolithic capillary column has been developed. The capillary monolithic column was prepared by in situ copolymerization of 2-(sulfooxy)ethyl methacrylate (SEMA) and ethylene dimethacrylate (EDMA) in the presence of porogens. The sulfate group provided by the monomer SEMA on the monolithic bed is used for the generation of the electroosmotic flow (EOF) from the anode to the cathode, but at the same time serves as a SCX stationary phase. A mixed-mode (RP/SCX) mechanism for separation of peptides was observed in the monolithic column, comprising hydrophobic and electrostatic interaction as well as electrophoretic migration at a low pH value of mobile phase. A column efficiency of more than 280000 plates/m for the unretained compound has been obtained on the prepared monoliths. The relative standard deviations observed for to and retention factors of peptides were about 0.32% and less than 0.71% for ten consecutive runs, respectively. Effects of mobile phase compositions on the EOF of the monolithic column and on the separation of peptides were investigated. The selectivity on separation of peptides in the monolithic capillary column could be easily manipulated by varying the mobile phase composition.
关键词[WOS]: ELECTROSPRAY-MASS-SPECTROMETRY ;  POROUS POLYMER MONOLITHS ;  LIQUID-CHROMATOGRAPHY ;  STATIONARY PHASES ;  AMINO-ACIDS ;  MEDIA ;  CHEMISTRY ;  PROTEINS ;  DRIVEN ;  FLOW
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000175693700006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/83221
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Wu, RA,Zou, HF,Fu, HJ,et al. Separation of peptides on mixed mode of reversed-phase and ion-exchange capillary electrochromatography with a monolithic column[J]. ELECTROPHORESIS,2002,23(9):1239-1245.
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