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Study of competitive binding of enantiomers to protein by affinity capillary electrochromatography
Ye, ML; Zou, HF; Liu, Z; Wu, RN; Lei, ZD; Ni, JY
关键词Affinity Capillary Electrochromatography Enantiomers Drug-protein Interaction Bovine Serum Albumin
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2002-01-15
27期:3-4页:651-660
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]BOVINE SERUM-ALBUMIN ; CHROMATOGRAPHY ; SEPARATION ; TECHNOLOGY ; TRYPTOPHAN ; RETENTION
英文摘要Affinity capillary electrochromatography (CEC) with zonal elution method was used to probe the competitive interactions of enantiomers with protein. In this approach, a known concentration of a competing agent is continuously applied to a CEC column with bovine serum albumin (BSA) physically adsorbed on SAX packing while injections of a small amount of analyte are made. The binding sites of solutes on the BSA molecule were determined by the changes in the retention factors of the solutes resulted from the addition of competitive agent. By using D- or L-tryptophan as competitive agents and D-, L-tryptophan and benzoin enantiomers as injected analytes showed that BSA molecule has a primary site to strongly bind L-tryptophan, but D-tryptophan dose not bind at this site; D- and L-tryptophan share a weak binding site on the BSA molecule. Benzoin enantiomers do not share any binding sites with either D- or L-tryptophan. Non-chiral compounds of trichloroacetic acid and n-hexanoic acid were applied as the competitive agents to study the binding of warfarin enantiomers to BSA, it was observed that trichloroacetic acid and n-hexanoic acid had a same binding site for warfarin enantiomers binding to BSA molecule. (C) 2002 Elsevier Science B.V. All rights reserved.
语种英语
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WOS记录号WOS:000173430500028
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被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/83223
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Ye, ML,Zou, HF,Liu, Z,et al. Study of competitive binding of enantiomers to protein by affinity capillary electrochromatography[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2002,27(3-4):651-660.
APA Ye, ML,Zou, HF,Liu, Z,Wu, RN,Lei, ZD,&Ni, JY.(2002).Study of competitive binding of enantiomers to protein by affinity capillary electrochromatography.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,27(3-4),651-660.
MLA Ye, ML,et al."Study of competitive binding of enantiomers to protein by affinity capillary electrochromatography".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 27.3-4(2002):651-660.
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