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题名: Study of competitive binding of enantiomers to protein by affinity capillary electrochromatography
作者: Ye, ML;  Zou, HF;  Liu, Z;  Wu, RN;  Lei, ZD;  Ni, JY
关键词: affinity capillary electrochromatography ;  enantiomers ;  drug-protein interaction ;  bovine serum albumin
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2002-01-15
卷: 27, 期:3-4, 页:651-660
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: Affinity capillary electrochromatography (CEC) with zonal elution method was used to probe the competitive interactions of enantiomers with protein. In this approach, a known concentration of a competing agent is continuously applied to a CEC column with bovine serum albumin (BSA) physically adsorbed on SAX packing while injections of a small amount of analyte are made. The binding sites of solutes on the BSA molecule were determined by the changes in the retention factors of the solutes resulted from the addition of competitive agent. By using D- or L-tryptophan as competitive agents and D-, L-tryptophan and benzoin enantiomers as injected analytes showed that BSA molecule has a primary site to strongly bind L-tryptophan, but D-tryptophan dose not bind at this site; D- and L-tryptophan share a weak binding site on the BSA molecule. Benzoin enantiomers do not share any binding sites with either D- or L-tryptophan. Non-chiral compounds of trichloroacetic acid and n-hexanoic acid were applied as the competitive agents to study the binding of warfarin enantiomers to BSA, it was observed that trichloroacetic acid and n-hexanoic acid had a same binding site for warfarin enantiomers binding to BSA molecule. (C) 2002 Elsevier Science B.V. All rights reserved.
关键词[WOS]: BOVINE SERUM-ALBUMIN ;  CHROMATOGRAPHY ;  SEPARATION ;  TECHNOLOGY ;  TRYPTOPHAN ;  RETENTION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000173430500028
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/83223
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Ye, ML,Zou, HF,Liu, Z,et al. Study of competitive binding of enantiomers to protein by affinity capillary electrochromatography[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2002,27(3-4):651-660.
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