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题名: Characteristics of electroosmotic flow and migration of neutral solutes under stepwise gradient elution of capillary electrochromatography
作者: Zhang, LH;  Zhang, WB;  Ping, GC;  Zhang, YK;  Kettrup, A
关键词: capillary electrochromatography ;  electroosmotic flow ;  retention of neutral solutes ;  stepwise gradient elution
刊名: ELECTROPHORESIS
发表日期: 2002-08-01
卷: 23, 期:15, 页:2417-2423
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A theoretical study on the velocity of electroosmotic flow (EOF) and the retention times of neutral solutes under multiple-step gradient of capillary electrochromatography (CEC) was carried out, focusing on that with three kinds of mobile phases. Through the model computations, the detaining time of the second kind of mobile phase in the column was proved to play an important role in affecting EOF. The variation speed of EOF was shown to be determined by the differences among dead times in different steps. In addition, the prediction of the retention times of 13 aromatic compounds under gradient mode was performed with the deduced equations. A relative error below 3.3% between the calculated and experimental values was obtained, which demonstrated the rationality of the theoretical deduction. Our study could not only improve the comprehension of stepwise gradient elution, but also be of significance for the further optimization of separation conditions in the analysis of complex samples.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  STATIONARY PHASES ;  REVERSED-PHASE ;  SEPARATION ;  ELECTROPHORESIS ;  PROTEINS ;  TEMPERATURE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000177526400010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/83251
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Inst Ecol Chem, GSF Natl Res Ctr Environm & Hlth, Oberschleissheim, Germany

Recommended Citation:
Zhang, LH,Zhang, WB,Ping, GC,et al. Characteristics of electroosmotic flow and migration of neutral solutes under stepwise gradient elution of capillary electrochromatography[J]. ELECTROPHORESIS,2002,23(15):2417-2423.
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