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题名: Development of a precolumn derivatization method for the determination of free amines in wastewater by high-performance liquid chromatography via fluorescent detection with 9-(2-hydroxyethyl)acridone
作者: You, JM;  Zhang, WB;  Zhang, QH;  Zhang, L;  Yan, C;  Zhang, YK
刊名: ANALYTICAL CHEMISTRY
发表日期: 2002
DOI: 10.1021/ac010285d
卷: 74, 期:1, 页:261-269
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A simple, sensitive, and mild method for the determination of amino compounds based on a condensation reaction with fluorescence detection has been developed. 9-(2-Hydroxyethyl)acridone reacts with coupling agent N,N-carbonyldiimidazole at ambient temperature to form activated amide intermediate 9-(2-acridone)oxyethylcarbonylimidazole (AOCD). The amide intermediate (AOCD) preferably reacts with amino compounds under mild reactions in the presence of 4-(dimethylamino)pyridine (base catalyst) in acetonitrile to give the corresponding sensitively fluorescent derivatives with an excitation maximum lambda(ex) 404 mn and an emission maximum at lambda(em) 440 nm. The labeled derivatives exhibit high stability under reversed-phase conditions. The fluorescence intensities of derivatives in various solvents or at different temperatures were investigated. The method, in conjunction with a gradient elution, offers a baseline resolution of the common amine derivatives on a reversed-phase C-18 column. The LC separation for the derivatized amines shows good reproducibility with acetonitrile-water including 2.5% DMF as mobile phase. The relative standard deviations (n = 6) for each amine derivative are <4.5%. The detection limits (at a signal-to-noise ratio of 3) per injection were 0.16-12.8 ng/mL. Further research for the field of application, based on the AOCD amide intermediate as derivatization reagent, for the determination of free amines in real water samples is achieved.
关键词[WOS]: CAPILLARY ZONE ELECTROPHORESIS ;  2-(9-ANTHRYL)ETHYL CHLOROFORMATE ;  FLUOROMETRIC DETECTION ;  ALIPHATIC-AMINES ;  N-NITROSOAMINES ;  ACID ANALYSIS ;  CHLORIDE ;  REAGENT ;  HYDROLYSATE ;  AGENT
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000173086200045
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/83265
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Acad Sinica, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Qufu Normal Univ, Dept Chem, Qufu 273165, Peoples R China

Recommended Citation:
You, JM,Zhang, WB,Zhang, QH,et al. Development of a precolumn derivatization method for the determination of free amines in wastewater by high-performance liquid chromatography via fluorescent detection with 9-(2-hydroxyethyl)acridone[J]. ANALYTICAL CHEMISTRY,2002,74(1):261-269.
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