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Development of a precolumn derivatization method for the determination of free amines in wastewater by high-performance liquid chromatography via fluorescent detection with 9-(2-hydroxyethyl)acridone
You, JM; Zhang, WB; Zhang, QH; Zhang, L; Yan, C; Zhang, YK
Source PublicationANALYTICAL CHEMISTRY
2002
DOI10.1021/ac010285d
Volume74Issue:1Pages:261-269
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordCAPILLARY ZONE ELECTROPHORESIS ; 2-(9-ANTHRYL)ETHYL CHLOROFORMATE ; FLUOROMETRIC DETECTION ; ALIPHATIC-AMINES ; N-NITROSOAMINES ; ACID ANALYSIS ; CHLORIDE ; REAGENT ; HYDROLYSATE ; AGENT
AbstractA simple, sensitive, and mild method for the determination of amino compounds based on a condensation reaction with fluorescence detection has been developed. 9-(2-Hydroxyethyl)acridone reacts with coupling agent N,N-carbonyldiimidazole at ambient temperature to form activated amide intermediate 9-(2-acridone)oxyethylcarbonylimidazole (AOCD). The amide intermediate (AOCD) preferably reacts with amino compounds under mild reactions in the presence of 4-(dimethylamino)pyridine (base catalyst) in acetonitrile to give the corresponding sensitively fluorescent derivatives with an excitation maximum lambda(ex) 404 mn and an emission maximum at lambda(em) 440 nm. The labeled derivatives exhibit high stability under reversed-phase conditions. The fluorescence intensities of derivatives in various solvents or at different temperatures were investigated. The method, in conjunction with a gradient elution, offers a baseline resolution of the common amine derivatives on a reversed-phase C-18 column. The LC separation for the derivatized amines shows good reproducibility with acetonitrile-water including 2.5% DMF as mobile phase. The relative standard deviations (n = 6) for each amine derivative are <4.5%. The detection limits (at a signal-to-noise ratio of 3) per injection were 0.16-12.8 ng/mL. Further research for the field of application, based on the AOCD amide intermediate as derivatization reagent, for the determination of free amines in real water samples is achieved.
Language英语
URL查看原文
WOS IDWOS:000173086200045
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Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/83265
Collection中国科学院大连化学物理研究所
Affiliation1.Acad Sinica, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Qufu Normal Univ, Dept Chem, Qufu 273165, Peoples R China
Recommended Citation
GB/T 7714
You, JM,Zhang, WB,Zhang, QH,et al. Development of a precolumn derivatization method for the determination of free amines in wastewater by high-performance liquid chromatography via fluorescent detection with 9-(2-hydroxyethyl)acridone[J]. ANALYTICAL CHEMISTRY,2002,74(1):261-269.
APA You, JM,Zhang, WB,Zhang, QH,Zhang, L,Yan, C,&Zhang, YK.(2002).Development of a precolumn derivatization method for the determination of free amines in wastewater by high-performance liquid chromatography via fluorescent detection with 9-(2-hydroxyethyl)acridone.ANALYTICAL CHEMISTRY,74(1),261-269.
MLA You, JM,et al."Development of a precolumn derivatization method for the determination of free amines in wastewater by high-performance liquid chromatography via fluorescent detection with 9-(2-hydroxyethyl)acridone".ANALYTICAL CHEMISTRY 74.1(2002):261-269.
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