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Study on the multiple sites binding of human serum albumin and porphyrin by affinity capillary electrophoresis
Zhang, WB; Zhang, LH; Ping, GC; Zhang, YK; Kettrup, A
关键词Affinity Capillary Electrophoresis Protein Binding Human Serum Albumin Porphyrin
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2002-02-25
768期:1页:211-214
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CHROMATOGRAPHY ; THERMODYNAMICS ; HEMOPEXIN ; DRUG
英文摘要Equations to describe the two sites binding between proteins and ligands were deduced. According to these equations, not only the binding constants, but also the mole fraction of proteins in different forms could be obtained. Using the published data on the interaction between human serum albumin (HSA) and three kinds of porphyrin (coproporphyrin (CP), uroporphyrin I (UP) and protoporphyrin (PP)), a further study on their binding was carried out. It was concluded that there may exist two binding sites with the binding constants at the first site. proved to be the preferential one, being 6.50 x 10(5) 1.94 x 10(6) and 8.94 x 10(5). respectively. In addition. it was also demonstrated that the two binding sites of HSA with CP and UP might be of different kinds, though those of HSA and PP were of the same kind but at different positions. (C) 2002 Elsevier Science B.V. All rights reserved.
语种英语
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WOS记录号WOS:000173925700021
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被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/83291
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.GSF, Natl Res Ctr Environm & Hlth, Inst Ecol Chem, D-85764 Neuherberg, Germany
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GB/T 7714
Zhang, WB,Zhang, LH,Ping, GC,et al. Study on the multiple sites binding of human serum albumin and porphyrin by affinity capillary electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2002,768(1):211-214.
APA Zhang, WB,Zhang, LH,Ping, GC,Zhang, YK,&Kettrup, A.(2002).Study on the multiple sites binding of human serum albumin and porphyrin by affinity capillary electrophoresis.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,768(1),211-214.
MLA Zhang, WB,et al."Study on the multiple sites binding of human serum albumin and porphyrin by affinity capillary electrophoresis".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 768.1(2002):211-214.
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