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Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix
Wang, H; Qin, JH; Dai, ZP; Wang, L; Bai, JL; Lin, BC
关键词Low Viscosity Dna Electrophoresis Microchip
刊名JOURNAL OF SEPARATION SCIENCE
2003-07-01
DOI10.1002/jssc.200301360
26期:9-10页:869-874
收录类别SCI
文章类型Article
部门归属1807
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]ENTANGLED POLYMER-SOLUTIONS ; TANDEM REPEAT LOCI ; CAPILLARY-ELECTROPHORESIS ; DNA SEPARATIONS ; MUTATIONS
英文摘要A novel protocol has been established to separate dsDNA fragments with high efficiency on glass chips by using an ultralow viscosity sieving matrix with added glucose. Low-molecular-weight hydroxypropylmethylcellulose (HPMC), with a viscosity nearly equivalent to that of water, was used to electrophoretically separate fluorescent inter-calator-labeled double-stranded DNA (dsDNA) fragments on microfluidic glass chips. In comparison with conventional sieving protocols, low-molecular-weight HPMC as sieving matrix could result in reduced running cost and analysis time, in addition to a comparable separation efficiency of dsDNA fragments. In this paper, the addition of glucose was investigated to enhance the separation of DNA in the lowest viscosity polymer evaluated. The effect of staining dye and field strength were also evaluated. At an applied electric field strength of 200 V/cm, satisfactory resolution of the PBR322/HaeIII DNA marker could be achieved within 4 min by using 2% HPMC-5 with 6% glucose added. Coelectrophoresing PCR product along with phiX174/HaeIII DNA sizing marker was also demonstrated by using the ultralow viscosity HPMC-5 solution on a glass chip.
语种英语
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WOS记录号WOS:000184392100017
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/83395
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
Wang, H,Qin, JH,Dai, ZP,et al. Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix[J]. JOURNAL OF SEPARATION SCIENCE,2003,26(9-10):869-874.
APA Wang, H,Qin, JH,Dai, ZP,Wang, L,Bai, JL,&Lin, BC.(2003).Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix.JOURNAL OF SEPARATION SCIENCE,26(9-10),869-874.
MLA Wang, H,et al."Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix".JOURNAL OF SEPARATION SCIENCE 26.9-10(2003):869-874.
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