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Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix
Wang, H; Qin, JH; Dai, ZP; Wang, L; Bai, JL; Lin, BC
KeywordLow Viscosity Dna Electrophoresis Microchip
Source PublicationJOURNAL OF SEPARATION SCIENCE
2003-07-01
DOI10.1002/jssc.200301360
Volume26Issue:9-10Pages:869-874
Indexed BySCI
SubtypeArticle
Department1807
Funding Project1807
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordENTANGLED POLYMER-SOLUTIONS ; TANDEM REPEAT LOCI ; CAPILLARY-ELECTROPHORESIS ; DNA SEPARATIONS ; MUTATIONS
AbstractA novel protocol has been established to separate dsDNA fragments with high efficiency on glass chips by using an ultralow viscosity sieving matrix with added glucose. Low-molecular-weight hydroxypropylmethylcellulose (HPMC), with a viscosity nearly equivalent to that of water, was used to electrophoretically separate fluorescent inter-calator-labeled double-stranded DNA (dsDNA) fragments on microfluidic glass chips. In comparison with conventional sieving protocols, low-molecular-weight HPMC as sieving matrix could result in reduced running cost and analysis time, in addition to a comparable separation efficiency of dsDNA fragments. In this paper, the addition of glucose was investigated to enhance the separation of DNA in the lowest viscosity polymer evaluated. The effect of staining dye and field strength were also evaluated. At an applied electric field strength of 200 V/cm, satisfactory resolution of the PBR322/HaeIII DNA marker could be achieved within 4 min by using 2% HPMC-5 with 6% glucose added. Coelectrophoresing PCR product along with phiX174/HaeIII DNA sizing marker was also demonstrated by using the ultralow viscosity HPMC-5 solution on a glass chip.
Language英语
URL查看原文
WOS IDWOS:000184392100017
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/83395
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wang, H,Qin, JH,Dai, ZP,et al. Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix[J]. JOURNAL OF SEPARATION SCIENCE,2003,26(9-10):869-874.
APA Wang, H,Qin, JH,Dai, ZP,Wang, L,Bai, JL,&Lin, BC.(2003).Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix.JOURNAL OF SEPARATION SCIENCE,26(9-10),869-874.
MLA Wang, H,et al."Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix".JOURNAL OF SEPARATION SCIENCE 26.9-10(2003):869-874.
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