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题名: Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix
作者: Wang, H;  Qin, JH;  Dai, ZP;  Wang, L;  Bai, JL;  Lin, BC
关键词: low viscosity ;  DNA ;  electrophoresis microchip
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2003-07-01
DOI: 10.1002/jssc.200301360
卷: 26, 期:9-10, 页:869-874
收录类别: SCI
文章类型: Article
部门归属: 1807
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A novel protocol has been established to separate dsDNA fragments with high efficiency on glass chips by using an ultralow viscosity sieving matrix with added glucose. Low-molecular-weight hydroxypropylmethylcellulose (HPMC), with a viscosity nearly equivalent to that of water, was used to electrophoretically separate fluorescent inter-calator-labeled double-stranded DNA (dsDNA) fragments on microfluidic glass chips. In comparison with conventional sieving protocols, low-molecular-weight HPMC as sieving matrix could result in reduced running cost and analysis time, in addition to a comparable separation efficiency of dsDNA fragments. In this paper, the addition of glucose was investigated to enhance the separation of DNA in the lowest viscosity polymer evaluated. The effect of staining dye and field strength were also evaluated. At an applied electric field strength of 200 V/cm, satisfactory resolution of the PBR322/HaeIII DNA marker could be achieved within 4 min by using 2% HPMC-5 with 6% glucose added. Coelectrophoresing PCR product along with phiX174/HaeIII DNA sizing marker was also demonstrated by using the ultralow viscosity HPMC-5 solution on a glass chip.
关键词[WOS]: ENTANGLED POLYMER-SOLUTIONS ;  TANDEM REPEAT LOCI ;  CAPILLARY-ELECTROPHORESIS ;  DNA SEPARATIONS ;  MUTATIONS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000184392100017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/83395
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Wang, H,Qin, JH,Dai, ZP,et al. Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix[J]. JOURNAL OF SEPARATION SCIENCE,2003,26(9-10):869-874.
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