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题名: On-line hyphenation of capillary isoelectric focusing and capillary gel electrophoresis by a dialysis interface
作者: Yang, C;  Liu, HC;  Yang, Q;  Zhang, LY;  Zhang, WB;  Zhang, YK
刊名: ANALYTICAL CHEMISTRY
发表日期: 2003-01-15
DOI: 10.1021/ac026187p
卷: 75, 期:2, 页:215-218
收录类别: SCI
文章类型: Article
部门归属: 1810
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: An on-line two-dimensional (2D) capillary electrophoresis (CE) system consisting of capillary isoelectric focusing (CIEF) and capillary gel electrophoresis (CGE) was introduced. To validate this 2D system, a dialysis interface was developed by mounting a hollow fiber on a methacrylate resin plate to hyphenate the two CE modes. The two dimensions of capillary shared a cathode fixated into a reservoir in the methacrylate plate; thus, with three electrodes and only one high-voltage source, a 2D CE framework was successfully established. A practical 2D CIEF-CGE experiment was carried out to deal with a target protein, hemoglobin (Hb). After the Hb variants with different isoelectric points (pIs) were focused in various bands in the first-dimension capillary, they were chemically mobilized one after another and fed to the second-dimension capillary for further separation in polyacrylamide gel. During this procedure, a single CIEF band was separated into several peaks due to different molecular weights. The resulting electrophoregrarn is quite different from that of either CIEF or CGE; therefore, more information about the studied Hb sample can be obtained.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ELECTROSPRAY MASS-SPECTROMETRY ;  ZONE-ELECTROPHORESIS ;  2-DIMENSIONAL SEPARATIONS ;  PEPTIDES ;  PROTEINS ;  ISOTACHOPHORESIS ;  ELECTROCHROMATOGRAPHY ;  MICRODIALYSIS ;  HEMOGLOBIN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000180430100006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/83425
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Yang, C,Liu, HC,Yang, Q,et al. On-line hyphenation of capillary isoelectric focusing and capillary gel electrophoresis by a dialysis interface[J]. ANALYTICAL CHEMISTRY,2003,75(2):215-218.
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