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On-line hyphenation of capillary isoelectric focusing and capillary gel electrophoresis by a dialysis interface
Yang, C; Liu, HC; Yang, Q; Zhang, LY; Zhang, WB; Zhang, YK
刊名ANALYTICAL CHEMISTRY
2003-01-15
DOI10.1021/ac026187p
75期:2页:215-218
收录类别SCI
文章类型Article
部门归属1810
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; ELECTROSPRAY MASS-SPECTROMETRY ; ZONE-ELECTROPHORESIS ; 2-DIMENSIONAL SEPARATIONS ; PEPTIDES ; PROTEINS ; ISOTACHOPHORESIS ; ELECTROCHROMATOGRAPHY ; MICRODIALYSIS ; HEMOGLOBIN
英文摘要An on-line two-dimensional (2D) capillary electrophoresis (CE) system consisting of capillary isoelectric focusing (CIEF) and capillary gel electrophoresis (CGE) was introduced. To validate this 2D system, a dialysis interface was developed by mounting a hollow fiber on a methacrylate resin plate to hyphenate the two CE modes. The two dimensions of capillary shared a cathode fixated into a reservoir in the methacrylate plate; thus, with three electrodes and only one high-voltage source, a 2D CE framework was successfully established. A practical 2D CIEF-CGE experiment was carried out to deal with a target protein, hemoglobin (Hb). After the Hb variants with different isoelectric points (pIs) were focused in various bands in the first-dimension capillary, they were chemically mobilized one after another and fed to the second-dimension capillary for further separation in polyacrylamide gel. During this procedure, a single CIEF band was separated into several peaks due to different molecular weights. The resulting electrophoregrarn is quite different from that of either CIEF or CGE; therefore, more information about the studied Hb sample can be obtained.
语种英语
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WOS记录号WOS:000180430100006
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被引频次:73[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/83425
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Yang, C,Liu, HC,Yang, Q,et al. On-line hyphenation of capillary isoelectric focusing and capillary gel electrophoresis by a dialysis interface[J]. ANALYTICAL CHEMISTRY,2003,75(2):215-218.
APA Yang, C,Liu, HC,Yang, Q,Zhang, LY,Zhang, WB,&Zhang, YK.(2003).On-line hyphenation of capillary isoelectric focusing and capillary gel electrophoresis by a dialysis interface.ANALYTICAL CHEMISTRY,75(2),215-218.
MLA Yang, C,et al."On-line hyphenation of capillary isoelectric focusing and capillary gel electrophoresis by a dialysis interface".ANALYTICAL CHEMISTRY 75.2(2003):215-218.
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