DICP OpenIR
Molecularly imprinted monolithic stationary phases for liquid chromatographic separation of enantiomers and diastereomers; Molecularly imprinted monolithic stationary phases for liquid chromatographic separation of enantiomers and diastereomers
Huang, XD; Zou, HF; Chen, XM; Luo, QZ; Kong, L
KeywordMolecular Imprinting Molecular Imprinting Stationary Phases Stationary Phases Monolithic Monolithic Lc Lc Enantiomer Separation Enantiomer Separation Diastereomer Separation Diastereomer Separation
Source PublicationJOURNAL OF CHROMATOGRAPHY A ; JOURNAL OF CHROMATOGRAPHY A
2003-01-17 ; 2003-01-17
Volume984Issue:2Pages:273-282
Indexed BySCI ; SCI
SubtypeArticle ; Article
Department1809 ; 1809
Funding Project1809 ; 1809
Contribution Rank1;1 ; 1;1
WOS HeadingsScience & Technology ; Science & Technology ; Life Sciences & Biomedicine ; Life Sciences & Biomedicine ; Physical Sciences ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Biochemical Research Methods ; Chemistry, Analytical ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Biochemistry & Molecular Biology ; Chemistry ; Chemistry
WOS KeywordCAPILLARY ELECTROCHROMATOGRAPHY ; CAPILLARY ELECTROCHROMATOGRAPHY ; SUSPENSION POLYMERIZATION ; SUSPENSION POLYMERIZATION ; CHIRAL RECOGNITION ; CHIRAL RECOGNITION ; SOLUTE RETENTION ; SOLUTE RETENTION ; AFFINITY MEDIA ; AFFINITY MEDIA ; POLYMERS ; POLYMERS ; SELECTIVITY ; SELECTIVITY ; PERFLUOROCARBON ; PERFLUOROCARBON ; TEMPLATE ; TEMPLATE ; BEADS ; BEADS
AbstractThe method for preparation of molecularly imprinted monolithic stationary phase has been improved to achieve liquid chromatographic separation of enantiomers and diastereomers. By adopting low polar porogenic solvents of toluene and dodecanol and optimal polymerization conditions, the molecularly imprinted monolithic stationary phases with good flow-through properties and high resolution were prepared. Enantiomers of amino acid derivatives and diastereomers of cinchona alkaloids were completely resolved using the monolithic stationary phases. The influence of porogenic composition, monomer-template ratio and polymerization conditions on the chromatographic performance was investigated. Some chromatographic conditions such as the composition of the mobile phase and the temperature were characterized. Scanning electron microscopy showed that the molecularly imprinted monolithic stationary phase has a large through-pore structure to allow the mobile phase to flow through the column at very low backpressure. Accelerated separations of enantiomers and diastereomers were therefore achieved at elevated flow rates. Finally, the chiral recognition performance of the prepared stationary phase in aqueous media was investigated. Hydrophobic interaction, and ionic and/or hydrogen bonding interactions were proposed to be responsible for the recognition mechanism. (C) 2002 Elsevier Science B.V. All rights reserved.; The method for preparation of molecularly imprinted monolithic stationary phase has been improved to achieve liquid chromatographic separation of enantiomers and diastereomers. By adopting low polar porogenic solvents of toluene and dodecanol and optimal polymerization conditions, the molecularly imprinted monolithic stationary phases with good flow-through properties and high resolution were prepared. Enantiomers of amino acid derivatives and diastereomers of cinchona alkaloids were completely resolved using the monolithic stationary phases. The influence of porogenic composition, monomer-template ratio and polymerization conditions on the chromatographic performance was investigated. Some chromatographic conditions such as the composition of the mobile phase and the temperature were characterized. Scanning electron microscopy showed that the molecularly imprinted monolithic stationary phase has a large through-pore structure to allow the mobile phase to flow through the column at very low backpressure. Accelerated separations of enantiomers and diastereomers were therefore achieved at elevated flow rates. Finally, the chiral recognition performance of the prepared stationary phase in aqueous media was investigated. Hydrophobic interaction, and ionic and/or hydrogen bonding interactions were proposed to be responsible for the recognition mechanism. (C) 2002 Elsevier Science B.V. All rights reserved.
Language英语 ; 英语
URL查看原文 ; 查看原文
WOS IDWOS:000180309800013 ; WOS:000180309800013
Citation statistics
Cited Times:140[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/83449
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Huang, XD,Zou, HF,Chen, XM,et al. Molecularly imprinted monolithic stationary phases for liquid chromatographic separation of enantiomers and diastereomers, Molecularly imprinted monolithic stationary phases for liquid chromatographic separation of enantiomers and diastereomers[J]. JOURNAL OF CHROMATOGRAPHY A, JOURNAL OF CHROMATOGRAPHY A,2003, 2003,984, 984(2):273-282, 273-282.
APA Huang, XD,Zou, HF,Chen, XM,Luo, QZ,&Kong, L.(2003).Molecularly imprinted monolithic stationary phases for liquid chromatographic separation of enantiomers and diastereomers.JOURNAL OF CHROMATOGRAPHY A,984(2),273-282.
MLA Huang, XD,et al."Molecularly imprinted monolithic stationary phases for liquid chromatographic separation of enantiomers and diastereomers".JOURNAL OF CHROMATOGRAPHY A 984.2(2003):273-282.
Files in This Item:
File Name/Size DocType Version Access License
2003H9W3J56564.pdf(537KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huang, XD]'s Articles
[Zou, HF]'s Articles
[Chen, XM]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huang, XD]'s Articles
[Zou, HF]'s Articles
[Chen, XM]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huang, XD]'s Articles
[Zou, HF]'s Articles
[Chen, XM]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.