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Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis
Yang, C; Zhang, LY; Liu, HC; Zhang, WB; Zhang, YK
KeywordTwo-dimensional Capillary Electrophoresis Capillary Isoelectric Focusing Capillary Electrophoresis Dialysis Interface Electroosmotic Flow
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2003-11-07
DOI10.1016/j.chroma.2003.08.031
Volume1018Issue:1Pages:97-103
Indexed BySCI
SubtypeArticle
Department1810
Funding Project1810
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordBASIC-PROTEINS ; SEPARATION ; CHROMATOGRAPHY ; PEPTIDES ; ISOTACHOPHORESIS ; INTERFACE ; SYSTEM ; COLUMN ; MICRODIALYSIS ; CONFIGURATION
AbstractCapillary isoelectric focusing (cIEF) and capillary zone electrophoresis (CZE) was on-line hyphenated by a dialysis interface to achieve a 2D capillary electrophoresis (CE) system. The system was used with just one high-voltage power supply and three electrodes (one cathode shared by the two dimensions). The focused zone in the first dimension (i.e. the cIEF) was driven to the dialysis interface by electroosmotic flow (EOF), besides chemical mobilization from the first anode to the shared cathode. And then in the second dimension (i.e. the CZE), the separated zone was further separated and driven by an inverted EOF, which originated from the charged layer of a cationic surfactant adsorbed onto the inner wall of the capillary. Finally, a solution of ribonuclease was rapidly separated to assess the feasibility of the two-dimensional CE implement. (C) 2003 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000185856700010
Citation statistics
Cited Times:41[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/83487
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Yang, C,Zhang, LY,Liu, HC,et al. Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY A,2003,1018(1):97-103.
APA Yang, C,Zhang, LY,Liu, HC,Zhang, WB,&Zhang, YK.(2003).Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis.JOURNAL OF CHROMATOGRAPHY A,1018(1),97-103.
MLA Yang, C,et al."Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis".JOURNAL OF CHROMATOGRAPHY A 1018.1(2003):97-103.
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