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Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis
Yang, C; Zhang, LY; Liu, HC; Zhang, WB; Zhang, YK
关键词Two-dimensional Capillary Electrophoresis Capillary Isoelectric Focusing Capillary Electrophoresis Dialysis Interface Electroosmotic Flow
刊名JOURNAL OF CHROMATOGRAPHY A
2003-11-07
DOI10.1016/j.chroma.2003.08.031
1018期:1页:97-103
收录类别SCI
文章类型Article
部门归属1810
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]BASIC-PROTEINS ; SEPARATION ; CHROMATOGRAPHY ; PEPTIDES ; ISOTACHOPHORESIS ; INTERFACE ; SYSTEM ; COLUMN ; MICRODIALYSIS ; CONFIGURATION
英文摘要Capillary isoelectric focusing (cIEF) and capillary zone electrophoresis (CZE) was on-line hyphenated by a dialysis interface to achieve a 2D capillary electrophoresis (CE) system. The system was used with just one high-voltage power supply and three electrodes (one cathode shared by the two dimensions). The focused zone in the first dimension (i.e. the cIEF) was driven to the dialysis interface by electroosmotic flow (EOF), besides chemical mobilization from the first anode to the shared cathode. And then in the second dimension (i.e. the CZE), the separated zone was further separated and driven by an inverted EOF, which originated from the charged layer of a cationic surfactant adsorbed onto the inner wall of the capillary. Finally, a solution of ribonuclease was rapidly separated to assess the feasibility of the two-dimensional CE implement. (C) 2003 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000185856700010
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被引频次:40[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/83487
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Yang, C,Zhang, LY,Liu, HC,et al. Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY A,2003,1018(1):97-103.
APA Yang, C,Zhang, LY,Liu, HC,Zhang, WB,&Zhang, YK.(2003).Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis.JOURNAL OF CHROMATOGRAPHY A,1018(1),97-103.
MLA Yang, C,et al."Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis".JOURNAL OF CHROMATOGRAPHY A 1018.1(2003):97-103.
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