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题名: Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis
作者: Yang, C;  Zhang, LY;  Liu, HC;  Zhang, WB;  Zhang, YK
关键词: two-dimensional capillary electrophoresis ;  capillary isoelectric focusing ;  capillary electrophoresis ;  dialysis interface ;  electroosmotic flow
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2003-11-07
DOI: 10.1016/j.chroma.2003.08.031
卷: 1018, 期:1, 页:97-103
收录类别: SCI
文章类型: Article
部门归属: 1810
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Capillary isoelectric focusing (cIEF) and capillary zone electrophoresis (CZE) was on-line hyphenated by a dialysis interface to achieve a 2D capillary electrophoresis (CE) system. The system was used with just one high-voltage power supply and three electrodes (one cathode shared by the two dimensions). The focused zone in the first dimension (i.e. the cIEF) was driven to the dialysis interface by electroosmotic flow (EOF), besides chemical mobilization from the first anode to the shared cathode. And then in the second dimension (i.e. the CZE), the separated zone was further separated and driven by an inverted EOF, which originated from the charged layer of a cationic surfactant adsorbed onto the inner wall of the capillary. Finally, a solution of ribonuclease was rapidly separated to assess the feasibility of the two-dimensional CE implement. (C) 2003 Elsevier B.V. All rights reserved.
关键词[WOS]: BASIC-PROTEINS ;  SEPARATION ;  CHROMATOGRAPHY ;  PEPTIDES ;  ISOTACHOPHORESIS ;  INTERFACE ;  SYSTEM ;  COLUMN ;  MICRODIALYSIS ;  CONFIGURATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000185856700010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/83487
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Yang, C,Zhang, LY,Liu, HC,et al. Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY A,2003,1018(1):97-103.
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