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题名: Pressurized capillary electrochromatography separation of peptides with strong cation exchange and hydrophilic interaction
作者: Zhang, K;  Gao, RY;  Jiang, ZJ;  Yao, CY;  Zhang, ZC;  Wang, QS;  Yan, C
关键词: capillary electrochromatography (CEC) ;  pressurized capillary electrochromatography ;  (pCEC) ;  hydrophilic chromatography ;  peptides
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2003-10-01
DOI: 10.1002/jssc.200301590
卷: 26, 期:15-16, 页:1389-1394
收录类别: SCI
文章类型: Article
部门归属: 1810
项目归属: 1810
产权排名: 2;7
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A pressurized capillary electrochromatography (pCEC) instrument with solvent gradient capability has been used for the separation of a peptide mixture. Retention mechanism and selectivity of the peptides were studied by pCEC using a strong cation exchange (SCX) column. The effects of applied voltage, supplementary pressure, organic modifier concentration, ionic strength,, and pH value on pCEC separation were investigated. It was found that the retention mechanism of the peptides in this system is based on a mixed mode of hydrophilic interaction, strong cation exchange, and electrophoresis. Compared with the separation results obtained by reverse phase pCEC and capillary electrophoresis (CE), this mixed-mode pCEC is more powerful for the separation of hydrophilic peptides with similar charge-to-mass ratio.
关键词[WOS]: LIQUID-CHROMATOGRAPHY ;  BONDED SILICA ;  COLUMNS ;  ELECTROPHORESIS ;  DERIVATIVES ;  RETENTION ;  PROTEINS ;  DETECTOR ;  PHASES ;  FLOW
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000186375800011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/83491
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, State Key Lab Elemento Organ Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116011, Peoples R China
3.Unimicro Technol Inc, Pleasanton, CA 94566 USA

Recommended Citation:
Zhang, K,Gao, RY,Jiang, ZJ,et al. Pressurized capillary electrochromatography separation of peptides with strong cation exchange and hydrophilic interaction[J]. JOURNAL OF SEPARATION SCIENCE,2003,26(15-16):1389-1394.
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