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Pressurized capillary electrochromatography separation of peptides with strong cation exchange and hydrophilic interaction
Zhang, K; Gao, RY; Jiang, ZJ; Yao, CY; Zhang, ZC; Wang, QS; Yan, C
关键词Capillary Electrochromatography (Cec) Pressurized Capillary Electrochromatography (Pcec) Hydrophilic Chromatography Peptides
刊名JOURNAL OF SEPARATION SCIENCE
2003-10-01
DOI10.1002/jssc.200301590
26期:15-16页:1389-1394
收录类别SCI
文章类型Article
部门归属1810
项目归属1810
产权排名2;7
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]LIQUID-CHROMATOGRAPHY ; BONDED SILICA ; COLUMNS ; ELECTROPHORESIS ; DERIVATIVES ; RETENTION ; PROTEINS ; DETECTOR ; PHASES ; FLOW
英文摘要A pressurized capillary electrochromatography (pCEC) instrument with solvent gradient capability has been used for the separation of a peptide mixture. Retention mechanism and selectivity of the peptides were studied by pCEC using a strong cation exchange (SCX) column. The effects of applied voltage, supplementary pressure, organic modifier concentration, ionic strength,, and pH value on pCEC separation were investigated. It was found that the retention mechanism of the peptides in this system is based on a mixed mode of hydrophilic interaction, strong cation exchange, and electrophoresis. Compared with the separation results obtained by reverse phase pCEC and capillary electrophoresis (CE), this mixed-mode pCEC is more powerful for the separation of hydrophilic peptides with similar charge-to-mass ratio.
语种英语
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WOS记录号WOS:000186375800011
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被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/83491
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, State Key Lab Elemento Organ Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116011, Peoples R China
3.Unimicro Technol Inc, Pleasanton, CA 94566 USA
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GB/T 7714
Zhang, K,Gao, RY,Jiang, ZJ,et al. Pressurized capillary electrochromatography separation of peptides with strong cation exchange and hydrophilic interaction[J]. JOURNAL OF SEPARATION SCIENCE,2003,26(15-16):1389-1394.
APA Zhang, K.,Gao, RY.,Jiang, ZJ.,Yao, CY.,Zhang, ZC.,...&Yan, C.(2003).Pressurized capillary electrochromatography separation of peptides with strong cation exchange and hydrophilic interaction.JOURNAL OF SEPARATION SCIENCE,26(15-16),1389-1394.
MLA Zhang, K,et al."Pressurized capillary electrochromatography separation of peptides with strong cation exchange and hydrophilic interaction".JOURNAL OF SEPARATION SCIENCE 26.15-16(2003):1389-1394.
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