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题名: Capillary electrochromatography for separation of peptides driven with electrophoretic mobility on monolithic column
作者: Wu, RA;  Zou, HF;  Ye, ML;  Lei, ZD;  Ni, JY
刊名: ANALYTICAL CHEMISTRY
发表日期: 2001-10-15
DOI: 10.1021/ac010413y
卷: 73, 期:20, 页:4918-4923
收录类别: SCI
文章类型: Article
部门归属: 18071
项目归属: 1809
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A mode of capillary electrochromatography for separation of ionic compounds driven by electrophoretic mobility on a neutrally hydrophobic monolithic column was developed. The monolithic column was prepared from the in situ copolymerization of lauryl methacrylate and ethylene dimethacrylate to form a C-12 hydrophobic stationary phase. It was found that EOF in this hydrophobic monolithic column was very poor, even the pH value of mobile phase at 8.0. The peptides at acidic buffer were separated on the basis of their differences in electrophoretic mobility and hydrophobic interaction with the stationary phase; therefore, different separation selectivity can be obtained in CEC from that in capillary zone electrophoresis (CZE). Separation of peptides has been realized with high column efficiency (up to 150 000 plates/meter) and good reproducibility (migration time with RSD < 0.5%), and all of the peptides, including some basic peptides, showed good peak symmetry. Effects of the mobile phase compositions on the retention of peptides at low pH have been investigated in a hydrophobic capillary monolithic column. The significant difference in selectivity of peptides in CZE and CEC has been observed. Some peptide isomers that cannot be separated by CZE have been successfully separated on the capillary monolithic column in this mode with the same buffer used.
关键词[WOS]: RIGID POLYMER MONOLITHS ;  LIQUID-CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  POROUS PROPERTIES ;  STATIONARY-PHASE ;  AMINO-ACIDS ;  CHEMISTRY ;  BASES ;  MEDIA
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000171696800020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/83737
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Wu, RA,Zou, HF,Ye, ML,et al. Capillary electrochromatography for separation of peptides driven with electrophoretic mobility on monolithic column[J]. ANALYTICAL CHEMISTRY,2001,73(20):4918-4923.
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