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题名: Affinity membrane chromatography for the analysis and purification of proteins
作者: Zou, HF;  Luo, QZ;  Zhou, DM
关键词: affinity chromatography ;  membrane stationary phases ;  monolithic columns ;  proteins
刊名: JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
发表日期: 2001-10-30
卷: 49, 期:1-3, 页:199-240
收录类别: SCI
文章类型: Review
部门归属: 18078
项目归属: 1809
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology ;  Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biophysics
英文摘要: Affinity chromatography is unique among separation methods as it is the only technique that permits the purification of proteins based on biological functions rather than individual physical or chemical properties. The high specificity of affinity chromatography is due to the strong interaction between the ligand and the proteins of interest. Membrane separation allows the processing of a large amount of sample in a relatively short time owing to its structure, which provides a system with rapid reaction kinetics. The integration of membrane and affinity chromatography provides a number of advantages over traditional affinity chromatography with porous-bead packed columns, especially with regard to time and recovery of activity. This review gives detailed descriptions of materials used as membrane substrates, preparation of basic membranes, coupling of affinity ligands to membrane supports, and categories of affinity membrane cartridges. It also summarizes the applications of cellulose/glycidyl methacrylate composite membranes for proteins separation developed in our laboratory. (C) 2001 Elsevier Science B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  HOLLOW-FIBER MEMBRANE ;  HYDROPHOBIC-INTERACTION CHROMATOGRAPHY ;  REVERSED-PHASE CHROMATOGRAPHY ;  CONTINUOUS POLYMER BEDS ;  CIBACRON-BLUE F3GA ;  IMMUNOGLOBULIN-G ;  ION-EXCHANGE ;  MACROPOROUS CHITOSAN ;  STATIONARY PHASES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000172468700013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/83739
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zou, HF,Luo, QZ,Zhou, DM. Affinity membrane chromatography for the analysis and purification of proteins[J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS,2001,49(1-3):199-240.
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