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题名: Migration of neutral solutes by double stepwise gradient elution in capillary electrochromatography
作者: Zhang, WB;  Zhang, LH;  Ping, GC;  Zhang, YK;  Kettrup, A
关键词: electrochromatography ;  gradient elution ;  electroosmotic flow
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2001-07-13
卷: 922, 期:1-2, 页:277-282
收录类别: SCI
文章类型: Article
部门归属: 18085
项目归属: 1810
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Characteristics of electroosmotic flow (EOF) and the migration of neutral solutes under double stepwise gradient elution in capillary electrochromatography were studied systematically. EOF velocity proved to be the function of operation time changing with the introduction of the second mobile phase. Accordingly, the retention of components also changed. The migration of neutral solutes was studied under the following three situations; A, components eluted when the column was filled only with the first kind of mobile phase; B, solutes eluted still in the first kind of mobile phase while at that time two kinds of mobile phase coexisted in the column and C, samples eluted in the second kind of mobile phase. Equations to describe the retention times of components under these three kinds of conditions were deduced and applied to predict the retention times of 12 aromatic compounds. Relative errors between experimental and calculated values were below 5.0%, which proved the reliability of the equations. In addition, parameters that might affect the retention time of solutes, such as the transferring time of mobile phase vials, the capacity factors of components and EOF velocities two steps were studied systematically (C) 2001 Elsevier Science B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ELECTROPHORESIS ;  SEPARATION ;  PROTEINS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000169975100026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/83755
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Dalian 116011, Peoples R China
2.GSF, Natl Res Ctr Environm & Hlth, Inst Ecol Chem, D-85764 Neuherberg, Germany

Recommended Citation:
Zhang, WB,Zhang, LH,Ping, GC,et al. Migration of neutral solutes by double stepwise gradient elution in capillary electrochromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2001,922(1-2):277-282.
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