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题名: Direct determination of sialic acids in serum by capillary electrophoresis with UV detection
作者: Dong, XL;  Xu, Y;  Lin, BC
关键词: capillary electrophoresis ;  sialic acids ;  human serum
刊名: CHROMATOGRAPHIA
发表日期: 2000-07-01
卷: 52, 期:1-2, 页:14-16
收录类别: SCI
文章类型: Article
部门归属: 4
项目归属: 401
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A novel method for the determination of N-acetylneuraminic acid (NANA) and N-glycolylneuraminic acid (NGNA) has been developed using high-performance capillary electrophoresis with UV detection at 195 nm, without pre or post-column derivatisation. The acids were separated in a 50-cm, fused-silica capillary (50 mu m i.d, 45.5-cm effective length) with Na2B4O7-Na2HPO4 buffer. The detection limit for NANA is a concentration of 9.6 x 10(-6) M or, in terms of mass: 3.879 x 10(-14) mol (39 fmol). This method is applicable to determination of NANA in normal human serum. The results were also compared with those of the colorimetrie method.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  N-GLYCOLYLNEURAMINIC ACIDS ;  LUNG-CANCER ;  ASSAY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000088514900002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84005
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Third Municipal Hosp, Dalian 116033, Peoples R China
3.Dalian Obstet & Gynecol Hosp, Dalian 116012, Peoples R China

Recommended Citation:
Dong, XL,Xu, Y,Lin, BC. Direct determination of sialic acids in serum by capillary electrophoresis with UV detection[J]. CHROMATOGRAPHIA,2000,52(1-2):14-16.
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