DICP OpenIR
Capillary electrophoretic study on UDP-sugars in cells
Xu, GW; Lehmann, R; Lu, X; Liebich, HM; Zhang, YK; Lu, PZ
关键词Cells Glucose Capillary Electrophoresis Udp-sugars Hexosamines
刊名SCIENCE IN CHINA SERIES B-CHEMISTRY
2000-02-01
43期:1页:85-93
收录类别SCI
文章类型Article
部门归属4
项目归属402
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; BLOOD-CELLS ; GRANULOCYTES ; UDPGALACTOSE ; NUCLEOTIDES ; SEPARATION ; METABOLISM ; UDPGLUCOSE ; GROWTH
英文摘要Glucose is an important regulator of cell growth and metabolism. Uridine diphosphate sugars (UDP-sugars), as the intermediate products of metabolism, play pivotal roles as precursors in the synthesis of complex carbohydrates and glycolipids as well as lectose. It is very important to study their metabolism in cells in clinical biochemistry. A capillary electrophoretic method has been developed for the analysis of UDP-sugars and nucleotides, By using an uncoated capillary (70cm x 50 mu m) and 20 mmol/L borax buffer (pH 9), 4 important UDP-sugars can be analyzed in 15 min at 22 kV with satisfactory precision and sensitivity. The developed method has been applied to analyze UDP-sugars concentrations in lymphocytes, fibroblasts and mesangial cells, and the results show it not only is much better than HPLC method, but also can be used to measure the energy charge of cells.
语种英语
原文出处查看原文
WOS记录号WOS:000085514600011
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/84017
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Chromatog, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Univ Tubingen, Med Klin, D-72076 Tubingen, Germany
推荐引用方式
GB/T 7714
Xu, GW,Lehmann, R,Lu, X,et al. Capillary electrophoretic study on UDP-sugars in cells[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2000,43(1):85-93.
APA Xu, GW,Lehmann, R,Lu, X,Liebich, HM,Zhang, YK,&Lu, PZ.(2000).Capillary electrophoretic study on UDP-sugars in cells.SCIENCE IN CHINA SERIES B-CHEMISTRY,43(1),85-93.
MLA Xu, GW,et al."Capillary electrophoretic study on UDP-sugars in cells".SCIENCE IN CHINA SERIES B-CHEMISTRY 43.1(2000):85-93.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
20005K96UO7Q3E.pdf(954KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xu, GW]的文章
[Lehmann, R]的文章
[Lu, X]的文章
百度学术
百度学术中相似的文章
[Xu, GW]的文章
[Lehmann, R]的文章
[Lu, X]的文章
必应学术
必应学术中相似的文章
[Xu, GW]的文章
[Lehmann, R]的文章
[Lu, X]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。