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题名: Capillary electrophoretic study on UDP-sugars in cells
作者: Xu, GW;  Lehmann, R;  Lu, X;  Liebich, HM;  Zhang, YK;  Lu, PZ
关键词: cells ;  glucose ;  capillary electrophoresis ;  UDP-sugars ;  hexosamines
刊名: SCIENCE IN CHINA SERIES B-CHEMISTRY
发表日期: 2000-02-01
卷: 43, 期:1, 页:85-93
收录类别: SCI
文章类型: Article
部门归属: 4
项目归属: 402
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Glucose is an important regulator of cell growth and metabolism. Uridine diphosphate sugars (UDP-sugars), as the intermediate products of metabolism, play pivotal roles as precursors in the synthesis of complex carbohydrates and glycolipids as well as lectose. It is very important to study their metabolism in cells in clinical biochemistry. A capillary electrophoretic method has been developed for the analysis of UDP-sugars and nucleotides, By using an uncoated capillary (70cm x 50 mu m) and 20 mmol/L borax buffer (pH 9), 4 important UDP-sugars can be analyzed in 15 min at 22 kV with satisfactory precision and sensitivity. The developed method has been applied to analyze UDP-sugars concentrations in lymphocytes, fibroblasts and mesangial cells, and the results show it not only is much better than HPLC method, but also can be used to measure the energy charge of cells.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  BLOOD-CELLS ;  GRANULOCYTES ;  UDPGALACTOSE ;  NUCLEOTIDES ;  SEPARATION ;  METABOLISM ;  UDPGLUCOSE ;  GROWTH
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000085514600011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84017
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Chromatog, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Univ Tubingen, Med Klin, D-72076 Tubingen, Germany

Recommended Citation:
Xu, GW,Lehmann, R,Lu, X,et al. Capillary electrophoretic study on UDP-sugars in cells[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2000,43(1):85-93.
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