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Capillary electrophoretic study on UDP-sugars in cells
Xu, GW; Lehmann, R; Lu, X; Liebich, HM; Zhang, YK; Lu, PZ
KeywordCells Glucose Capillary Electrophoresis Udp-sugars Hexosamines
Source PublicationSCIENCE IN CHINA SERIES B-CHEMISTRY
2000-02-01
Volume43Issue:1Pages:85-93
Indexed BySCI
SubtypeArticle
Department4
Funding Project402
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; BLOOD-CELLS ; GRANULOCYTES ; UDPGALACTOSE ; NUCLEOTIDES ; SEPARATION ; METABOLISM ; UDPGLUCOSE ; GROWTH
AbstractGlucose is an important regulator of cell growth and metabolism. Uridine diphosphate sugars (UDP-sugars), as the intermediate products of metabolism, play pivotal roles as precursors in the synthesis of complex carbohydrates and glycolipids as well as lectose. It is very important to study their metabolism in cells in clinical biochemistry. A capillary electrophoretic method has been developed for the analysis of UDP-sugars and nucleotides, By using an uncoated capillary (70cm x 50 mu m) and 20 mmol/L borax buffer (pH 9), 4 important UDP-sugars can be analyzed in 15 min at 22 kV with satisfactory precision and sensitivity. The developed method has been applied to analyze UDP-sugars concentrations in lymphocytes, fibroblasts and mesangial cells, and the results show it not only is much better than HPLC method, but also can be used to measure the energy charge of cells.
Language英语
URL查看原文
WOS IDWOS:000085514600011
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/84017
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Chromatog, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Univ Tubingen, Med Klin, D-72076 Tubingen, Germany
Recommended Citation
GB/T 7714
Xu, GW,Lehmann, R,Lu, X,et al. Capillary electrophoretic study on UDP-sugars in cells[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2000,43(1):85-93.
APA Xu, GW,Lehmann, R,Lu, X,Liebich, HM,Zhang, YK,&Lu, PZ.(2000).Capillary electrophoretic study on UDP-sugars in cells.SCIENCE IN CHINA SERIES B-CHEMISTRY,43(1),85-93.
MLA Xu, GW,et al."Capillary electrophoretic study on UDP-sugars in cells".SCIENCE IN CHINA SERIES B-CHEMISTRY 43.1(2000):85-93.
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