DICP OpenIR
Transient state of chiral recognition in a binary mixture of cyclodextrins in capillary electrophoresis
Zhu, XF; Ding, YS; Lin, BC; Jakob, A; Koppenhoefer, B
KeywordEnantiomer Separation Chiral Selectors Transient State Buffer Composition Cyclodextrins Aminoglutethimide
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2000-08-04
Volume888Issue:1-2Pages:241-250
Indexed BySCI
SubtypeArticle
Department4
Funding Project401
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordLIQUID-CHROMATOGRAPHIC SEPARATION ; DRUGS ; AMINOGLUTETHIMIDE ; AGENT ; ENANTIOMERS ; METABOLITE
AbstractThe transient state (as the defined point where no enantioseparation is obtained in a dual chiral selector system) of chiral recognition of aminoglutethimide in a binary mixture of neutral cyclodextrins (CDs) was studied by capillary electrophoresis (CE). The following three dual selector systems were used: alpha-cyclodextrin (alpha-CD) and beta-cyclodextrin (beta-CD); alpha-CD and heptakis(di-O-methyl-beta-cyclodextrin) (DM-beta-CD); alpha-CD and heptakis(tri-O-methyl-beta-cyclodextrin) (TM-beta-CD). The S-(-) enantiomer of the analyte was more strongly retained in the presence of either alpha-CD or TM-beta-CD at pH 2.5, 100 mM phosphate buffer, while the R-(+) enantiomer was more strongly retained in the presence of either P-CD or DM-P-CD. In the more simple case, the elution order is invariably kept if the enantiomers have the same elution order in either one of the two hosts of the binary mixture. In contrast, the elution order may be switched by varying the concentration ratio of two hosts that produce opposite elution order for this particular analyte. In such a dual selector system, the enantioselectivity will disappear at the transient state at a certain ratio of host,:host, Moreover, the migration times of the two enantiomers with host, alone (diluted in buffer) is approximately equal to the migration times at the corresponding concentration of host, alone (diluted in buffer), where the ratio of concentrations of host,:host, is the same as in the binary mixture at the transient state. As found by nuclear magnetic resonance experiments, the analyte is forming a 1:1 complex with either one of the CDs applied. From this finding, a theoretical model based on the mobility difference of the two enantiomers was derived that was used to simulate the transient state. (C) 2000 Elsevier Science B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000088414100024
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/84021
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Tubingen, Inst Organ Chem, D-72076 Tubingen, Germany
Recommended Citation
GB/T 7714
Zhu, XF,Ding, YS,Lin, BC,et al. Transient state of chiral recognition in a binary mixture of cyclodextrins in capillary electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY A,2000,888(1-2):241-250.
APA Zhu, XF,Ding, YS,Lin, BC,Jakob, A,&Koppenhoefer, B.(2000).Transient state of chiral recognition in a binary mixture of cyclodextrins in capillary electrophoresis.JOURNAL OF CHROMATOGRAPHY A,888(1-2),241-250.
MLA Zhu, XF,et al."Transient state of chiral recognition in a binary mixture of cyclodextrins in capillary electrophoresis".JOURNAL OF CHROMATOGRAPHY A 888.1-2(2000):241-250.
Files in This Item:
File Name/Size DocType Version Access License
200022EA6H73AB.pdf(776KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhu, XF]'s Articles
[Ding, YS]'s Articles
[Lin, BC]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhu, XF]'s Articles
[Ding, YS]'s Articles
[Lin, BC]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhu, XF]'s Articles
[Ding, YS]'s Articles
[Lin, BC]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.