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题名: Synthesis and characteristics of the human serum albumin-triazine chiral stationary phase
作者: Zhang, Q;  Zou, HF;  Chen, XM;  Wang, HL;  Luo, QZ;  Ni, JY
关键词: HSA chiral stationary phase ;  s-triazine activator ;  high-performance frontal analysis ;  resolution of enantiomers
刊名: CHIRALITY
发表日期: 2000
卷: 12, 期:10, 页:714-719
收录类别: SCI
文章类型: Article
部门归属: 4
项目归属: 403
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Chemistry, Medicinal ;  Chemistry, Analytical ;  Chemistry, Organic ;  Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy ;  Chemistry
英文摘要: Human serum albumin (HSA) was successfully bonded to silica with s-triazine as activator. The coupling reaction by this method was rapid and effective. The triazine-activated silica is relatively stable and can be installed for at least 1 month without obvious loss of reactivity when stored below 30 degreesC, pH below 7. It was observed that the amount of bound HSA reached 120 mg/g silica calculated from the UV absorbance difference of the HSA solution. d,l-tryptophan was selected as the probe solute to characterize the properties of HSA bonded s-triazine chiral stationary phase, and separation factor of 9.4 was obtained for d,l-tryptophan. Furthermore, the amount of effective HSA on silica was measured by high-performance frontal analysis, and only 16.8 mg/g silica was responsible for the resolution of d,l-tryptophan. These results indicate that the amount of both the bound and effective HSA on silica with triazine as activator was much higher than those by the Schiff base coupling method. Different kinds of enantiomers were resolved successfully on the aminopropylsilica-bonded HSA s-triazine chiral stationary phase. (C) 2000 Wiley-Liss, Inc.
关键词[WOS]: LIQUID-CHROMATOGRAPHIC SEPARATION ;  CELLULASE CBH-I ;  AFFINITY-CHROMATOGRAPHY ;  ENANTIOMERS ;  HPLC ;  RESOLUTION ;  BINDING ;  RECOGNITION ;  ENZYMES ;  COLUMN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000090086700003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84053
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zhang, Q,Zou, HF,Chen, XM,et al. Synthesis and characteristics of the human serum albumin-triazine chiral stationary phase[J]. CHIRALITY,2000,12(10):714-719.
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