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Synthesis and characteristics of the human serum albumin-triazine chiral stationary phase; Synthesis and characteristics of the human serum albumin-triazine chiral stationary phase
Zhang, Q; Zou, HF; Chen, XM; Wang, HL; Luo, QZ; Ni, JY
关键词Hsa Chiral Stationary Phase Hsa Chiral Stationary Phase S-triazine Activator S-triazine Activator High-performance Frontal Analysis High-performance Frontal Analysis Resolution Of Enantiomers Resolution Of Enantiomers
刊名CHIRALITY ; CHIRALITY
2000 ; 2000
12期:10页:714-719
收录类别SCI ; SCI
文章类型Article ; Article
部门归属4 ; 4
项目归属403 ; 403
WOS标题词Science & Technology ; Science & Technology ; Life Sciences & Biomedicine ; Life Sciences & Biomedicine ; Physical Sciences ; Physical Sciences
类目[WOS]Chemistry, Medicinal ; Chemistry, Medicinal ; Chemistry, Analytical ; Chemistry, Analytical ; Chemistry, Organic ; Chemistry, Organic ; Pharmacology & Pharmacy ; Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy ; Pharmacology & Pharmacy ; Chemistry ; Chemistry
关键词[WOS]LIQUID-CHROMATOGRAPHIC SEPARATION ; LIQUID-CHROMATOGRAPHIC SEPARATION ; CELLULASE CBH-I ; CELLULASE CBH-I ; AFFINITY-CHROMATOGRAPHY ; AFFINITY-CHROMATOGRAPHY ; ENANTIOMERS ; ENANTIOMERS ; HPLC ; HPLC ; RESOLUTION ; RESOLUTION ; BINDING ; BINDING ; RECOGNITION ; RECOGNITION ; ENZYMES ; ENZYMES ; COLUMN ; COLUMN
英文摘要Human serum albumin (HSA) was successfully bonded to silica with s-triazine as activator. The coupling reaction by this method was rapid and effective. The triazine-activated silica is relatively stable and can be installed for at least 1 month without obvious loss of reactivity when stored below 30 degreesC, pH below 7. It was observed that the amount of bound HSA reached 120 mg/g silica calculated from the UV absorbance difference of the HSA solution. d,l-tryptophan was selected as the probe solute to characterize the properties of HSA bonded s-triazine chiral stationary phase, and separation factor of 9.4 was obtained for d,l-tryptophan. Furthermore, the amount of effective HSA on silica was measured by high-performance frontal analysis, and only 16.8 mg/g silica was responsible for the resolution of d,l-tryptophan. These results indicate that the amount of both the bound and effective HSA on silica with triazine as activator was much higher than those by the Schiff base coupling method. Different kinds of enantiomers were resolved successfully on the aminopropylsilica-bonded HSA s-triazine chiral stationary phase. (C) 2000 Wiley-Liss, Inc.; Human serum albumin (HSA) was successfully bonded to silica with s-triazine as activator. The coupling reaction by this method was rapid and effective. The triazine-activated silica is relatively stable and can be installed for at least 1 month without obvious loss of reactivity when stored below 30 degreesC, pH below 7. It was observed that the amount of bound HSA reached 120 mg/g silica calculated from the UV absorbance difference of the HSA solution. d,l-tryptophan was selected as the probe solute to characterize the properties of HSA bonded s-triazine chiral stationary phase, and separation factor of 9.4 was obtained for d,l-tryptophan. Furthermore, the amount of effective HSA on silica was measured by high-performance frontal analysis, and only 16.8 mg/g silica was responsible for the resolution of d,l-tryptophan. These results indicate that the amount of both the bound and effective HSA on silica with triazine as activator was much higher than those by the Schiff base coupling method. Different kinds of enantiomers were resolved successfully on the aminopropylsilica-bonded HSA s-triazine chiral stationary phase. (C) 2000 Wiley-Liss, Inc.
语种英语 ; 英语
原文出处查看原文 ; 查看原文
WOS记录号WOS:000090086700003 ; WOS:000090086700003
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/84053
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Q,Zou, HF,Chen, XM,et al. Synthesis and characteristics of the human serum albumin-triazine chiral stationary phase, Synthesis and characteristics of the human serum albumin-triazine chiral stationary phase[J]. CHIRALITY, CHIRALITY,2000, 2000,12, 12(10):714-719, 714-719.
APA Zhang, Q,Zou, HF,Chen, XM,Wang, HL,Luo, QZ,&Ni, JY.(2000).Synthesis and characteristics of the human serum albumin-triazine chiral stationary phase.CHIRALITY,12(10),714-719.
MLA Zhang, Q,et al."Synthesis and characteristics of the human serum albumin-triazine chiral stationary phase".CHIRALITY 12.10(2000):714-719.
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