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Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis
He, XY; Xiao, HB; Liang, XM; Lin, BC
KeywordCapillary Zone Electrophoresis Interaction Puc19dna Ovalbumin Binding Constant
Source PublicationJOURNAL OF SEPARATION SCIENCE
2002-07-01
Volume25Issue:10-11Pages:711-714
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordBINDING ASSAYS ; DNA-BINDING ; PROTEIN ; TOOL
AbstractCapillary zone electrophoresis (CZE) was used to study the interaction between pUC19DNA (pUC19) and ovalbumin (Ova). Samples containing pUC19 and Ova at various ratios were incubated at room temperature and were then separated by CZE with tris-acetate buffer at pH 7.2. Reduction in ultraviolet (UV) absorbance of pUC19 was due to the decrease of free pUC19 after binding to Ova. The binding constant of the interaction calculated from the Scatchard plot was (1.46+/-0.15) x 10(5) M-1. The use of polyacrylamide-coated capillary showed better effects than that of uncoated capillary. The results show that it is important to keep a constant ionic strength in the samples in order to obtain accurate quantitative data in binding assays by CZE.
Language英语
URL查看原文
WOS IDWOS:000177594400012
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/84185
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
He, XY,Xiao, HB,Liang, XM,et al. Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis[J]. JOURNAL OF SEPARATION SCIENCE,2002,25(10-11):711-714.
APA He, XY,Xiao, HB,Liang, XM,&Lin, BC.(2002).Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis.JOURNAL OF SEPARATION SCIENCE,25(10-11),711-714.
MLA He, XY,et al."Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis".JOURNAL OF SEPARATION SCIENCE 25.10-11(2002):711-714.
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