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题名: Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis
作者: He, XY;  Xiao, HB;  Liang, XM;  Lin, BC
关键词: capillary zone electrophoresis ;  interaction ;  pUC19DNA ;  ovalbumin ;  binding constant
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2002-07-01
卷: 25, 期:10-11, 页:711-714
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Capillary zone electrophoresis (CZE) was used to study the interaction between pUC19DNA (pUC19) and ovalbumin (Ova). Samples containing pUC19 and Ova at various ratios were incubated at room temperature and were then separated by CZE with tris-acetate buffer at pH 7.2. Reduction in ultraviolet (UV) absorbance of pUC19 was due to the decrease of free pUC19 after binding to Ova. The binding constant of the interaction calculated from the Scatchard plot was (1.46+/-0.15) x 10(5) M-1. The use of polyacrylamide-coated capillary showed better effects than that of uncoated capillary. The results show that it is important to keep a constant ionic strength in the samples in order to obtain accurate quantitative data in binding assays by CZE.
关键词[WOS]: BINDING ASSAYS ;  DNA-BINDING ;  PROTEIN ;  TOOL
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000177594400012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84185
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
He, XY,Xiao, HB,Liang, XM,et al. Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis[J]. JOURNAL OF SEPARATION SCIENCE,2002,25(10-11):711-714.
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