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Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis
He, XY; Xiao, HB; Liang, XM; Lin, BC
关键词Capillary Zone Electrophoresis Interaction Puc19dna Ovalbumin Binding Constant
刊名JOURNAL OF SEPARATION SCIENCE
2002-07-01
25期:10-11页:711-714
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]BINDING ASSAYS ; DNA-BINDING ; PROTEIN ; TOOL
英文摘要Capillary zone electrophoresis (CZE) was used to study the interaction between pUC19DNA (pUC19) and ovalbumin (Ova). Samples containing pUC19 and Ova at various ratios were incubated at room temperature and were then separated by CZE with tris-acetate buffer at pH 7.2. Reduction in ultraviolet (UV) absorbance of pUC19 was due to the decrease of free pUC19 after binding to Ova. The binding constant of the interaction calculated from the Scatchard plot was (1.46+/-0.15) x 10(5) M-1. The use of polyacrylamide-coated capillary showed better effects than that of uncoated capillary. The results show that it is important to keep a constant ionic strength in the samples in order to obtain accurate quantitative data in binding assays by CZE.
语种英语
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WOS记录号WOS:000177594400012
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/84185
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
He, XY,Xiao, HB,Liang, XM,et al. Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis[J]. JOURNAL OF SEPARATION SCIENCE,2002,25(10-11):711-714.
APA He, XY,Xiao, HB,Liang, XM,&Lin, BC.(2002).Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis.JOURNAL OF SEPARATION SCIENCE,25(10-11),711-714.
MLA He, XY,et al."Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis".JOURNAL OF SEPARATION SCIENCE 25.10-11(2002):711-714.
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