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题名: Interaction between netropsin and double-stranded DNA in capillary zone electrophoresis and affinity capillary electrophoresis
作者: He, XY;  Li, DZ;  Liang, AY;  Lin, BC
关键词: affinity capillary electrophoresis ;  DNA ;  netropsin
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2002-12-27
卷: 982, 期:2, 页:285-291
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Capillary zone electrophoresis (CZE) and affinity capillary electrophoresis (ACE) were applied to study the interaction between netropsin and a 14mer double-stranded DNA (dsDNA). The use of a polyacrylamide coated capillary can suppress the electroosmotic flow (EOF) and the adsorption of DNA onto the wall. Better analysis of the DNA was achieved in a coated capillary upon Tris-acetate. In CZE, the peak width broadened due to the affinity interaction between dsDNA and netropsin. In ACE, o-toluic acid, a negatively charged molecule was used as the indicator to monitor the changes of EOF when netropsin was added to the running buffer. The 14mer dsDNA showed different mobilities upon various concentrations of netropsin due to the affinity interaction between the dsDNA and netropsin. The binding constants of this interaction were (1.07 +/- 0.10) . 10(5) M-1 calculated from CZE and (4.75 +/- 0.30) . 10(4) M-1 from ACE using a Scatchard plot. The binding stoichiometry was 1:1 calculated from CZE which was superior to ACE in this study. (C) 2002 Elsevier Science B.V. All rights reserved.
关键词[WOS]: RAMAN-SPECTROSCOPY ;  BINDING LIGANDS ;  MINOR-GROOVE ;  RECOGNITION ;  ACID ;  TOOL
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000179485200011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84203
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
He, XY,Li, DZ,Liang, AY,et al. Interaction between netropsin and double-stranded DNA in capillary zone electrophoresis and affinity capillary electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY A,2002,982(2):285-291.
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