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题名: Competing binding of metal ions with protein studied by microdialysis
作者: Guo, M;  Kong, L;  Mao, XQ;  Li, X;  Zou, HF
关键词: microdialysis ;  high performance liquid chromatography ;  human serum albumin ;  metal ions ;  competing binding
刊名: SCIENCE IN CHINA SERIES B-CHEMISTRY
发表日期: 2002-04-01
卷: 45, 期:2, 页:151-157
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A method has been established to study the competing binding of metal ions with protein by a combined technique of microdialysis with high performance liquid chromatography (HPLC). Ni2+, Cd2+, Zn2+, Cu2+ and human serum albumin (HSA) were chosen as model metal ions and protein. The experimental results show that Ni2+ and Cu2+ share a common primary binding site on HSA, and Zn2+ and Cd2+ share a different common primary binding site from them, but there is a common multi-metal binding site for all of those four metal ions. This method show advantages of fast sampling, easily to be operated and especially to be useful when ideal spectroscopic probes are not available for the study of interaction between protein and metal ions.
关键词[WOS]: HUMAN-SERUM-ALBUMIN ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  SITE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000175009000006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84213
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116011, Peoples R China
2.Dalian Univ, Dept Chem & Chem Technol, Dalian 116622, Peoples R China

Recommended Citation:
Guo, M,Kong, L,Mao, XQ,et al. Competing binding of metal ions with protein studied by microdialysis[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2002,45(2):151-157.
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