DICP OpenIR
Protein A immobilized monolithic capillary column for affinity chromatography
Pan, ZF; Zou, HF; Mo, WM; Huang, XD; Wu, RN
KeywordAffinity Chromatography Monolithic Rod Capillary Column Protein a Higg
Source PublicationANALYTICA CHIMICA ACTA
2002-08-21
Volume466Issue:1Pages:141-150
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; RIGID POLYMER MONOLITHS ; STATIONARY PHASES ; SEPARATION MEDIA ; POROUS PROPERTIES ; CONTINUOUS BEDS ; EXCHANGE CHROMATOGRAPHY ; MACROPOROUS POLYMERS ; ELECTROCHROMATOGRAPHY ; CHEMISTRY
AbstractMonolithic capillary columns for affinity chromatography were prepared by an in situ polymerization procedure using glycidyl methacrylate (GMA) as a monomer and trimethylolpropane trimethacrylate (TRIM) and ethylene dimethacrylate (EDMA) as cross-linkers, respectively. Scanning electron microscopy was applied to characterize the morphology of the end of monolithic capillary and mercury intrusion porosimetry to characterize the polymer rod prepared within the confines of a stainless steel column with 50 mm x 4.6 mm i.d. under the same polymerization condition. Obvious differences in the porous properties between the TRIM- and EDMA-based monoliths could be observed. Moreover, the mechanical stability of these two monolithic capillary columns was compared by testing the reproducibility of the column performance. The rod prepared with GMA and TRIM proved to be mechanically more stable than that prepared with GMA and EDMA. Protein A was immobilized on the monolithic rod for affinity chromatography and the experiments were performed on a capillary electrophoresis instrument, using its pressure system as the driving force. Non-specific adsorption was not observed on the TRIM-based affinity column, as proved with bovine serum albumin (BSA) as a test protein. The affinity column prepared with GMA and TRIM was then applied to determine the hIgG concentration in human serum. The correlative coefficient of the calibration curve reached 0.9942. The amount of adsorbed hIgG was unaffected by the flow rate of the loading buffer, which makes this method suitable for fast determination of biomacromolecules in microliter samples. (C) 2002 Elsevier Science B.V All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000177682900017
Citation statistics
Cited Times:68[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/84217
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Zhejiang Univ Technol, Coll Chem Engn, Zheijang 310014, Peoples R China
Recommended Citation
GB/T 7714
Pan, ZF,Zou, HF,Mo, WM,et al. Protein A immobilized monolithic capillary column for affinity chromatography[J]. ANALYTICA CHIMICA ACTA,2002,466(1):141-150.
APA Pan, ZF,Zou, HF,Mo, WM,Huang, XD,&Wu, RN.(2002).Protein A immobilized monolithic capillary column for affinity chromatography.ANALYTICA CHIMICA ACTA,466(1),141-150.
MLA Pan, ZF,et al."Protein A immobilized monolithic capillary column for affinity chromatography".ANALYTICA CHIMICA ACTA 466.1(2002):141-150.
Files in This Item:
File Name/Size DocType Version Access License
2002LGM6N9I30M.pdf(160KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Pan, ZF]'s Articles
[Zou, HF]'s Articles
[Mo, WM]'s Articles
Baidu academic
Similar articles in Baidu academic
[Pan, ZF]'s Articles
[Zou, HF]'s Articles
[Mo, WM]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Pan, ZF]'s Articles
[Zou, HF]'s Articles
[Mo, WM]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.