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Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis
Li, WJ; Zhong, Y; Lin, BC; Su, ZG
关键词Semi-aqueous Capillary Electrophoresis Capillary Electrophoresis Buffer Composition Proteins Poly(Ethylene Glycol)
刊名JOURNAL OF CHROMATOGRAPHY A
2001-01-05
905期:1-2页:299-307
收录类别SCI
文章类型Article
部门归属18035
项目归属1807
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]MODIFIED SUPEROXIDE-DISMUTASE ; MODIFIED LYSOZYME ; ASPARAGINASE ; RIBONUCLEASE ; ACTIVATION ; ENZYMES ; CHARGE
英文摘要A new program to characterize polyethylene glycol-modified (PEGylated) proteins is outlined using capillary zone electrophoresis (CZE). PEGylated ribonuclease A and lysozyme were selected as examples. Five separation procedures were compared to select out the mixed buffer of acetonitrile-water (1:1, v/v) at pH 2.5 as the best to characterize the PEGylated proteins without sample pretreatment. Polyethylene oxide (PEO) with a high molecular mass of 8X10(6) was applied to rinse the capillary to form a dynamic coating which would decrease the undesirable proteins adsorbed to the inner wall of the silica. The electroosmotic flow (EOF) mobility of the five procedures was determined, respectively. It is found that acetonitrile is mainly responsible for the good resolution of PEGylated proteins with the help of PEO coating in the semi-aqueous system. The low EOF mobility and current in the semi-aqueous system might also have some responsibility for the high resolution. The semi-aqueous procedure described in this paper also demonstrates higher resolution of natural proteins than aqueous ones. (C) 2001 Elsevier Science B.V. All rights reserved.
语种英语
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WOS记录号WOS:000166339600029
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被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/84611
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
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Li, WJ,Zhong, Y,Lin, BC,et al. Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY A,2001,905(1-2):299-307.
APA Li, WJ,Zhong, Y,Lin, BC,&Su, ZG.(2001).Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis.JOURNAL OF CHROMATOGRAPHY A,905(1-2),299-307.
MLA Li, WJ,et al."Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis".JOURNAL OF CHROMATOGRAPHY A 905.1-2(2001):299-307.
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