DICP OpenIR
Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis
Li, WJ; Zhong, Y; Lin, BC; Su, ZG
KeywordSemi-aqueous Capillary Electrophoresis Capillary Electrophoresis Buffer Composition Proteins Poly(Ethylene Glycol)
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2001-01-05
Volume905Issue:1-2Pages:299-307
Indexed BySCI
SubtypeArticle
Department18035
Funding Project1807
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordMODIFIED SUPEROXIDE-DISMUTASE ; MODIFIED LYSOZYME ; ASPARAGINASE ; RIBONUCLEASE ; ACTIVATION ; ENZYMES ; CHARGE
AbstractA new program to characterize polyethylene glycol-modified (PEGylated) proteins is outlined using capillary zone electrophoresis (CZE). PEGylated ribonuclease A and lysozyme were selected as examples. Five separation procedures were compared to select out the mixed buffer of acetonitrile-water (1:1, v/v) at pH 2.5 as the best to characterize the PEGylated proteins without sample pretreatment. Polyethylene oxide (PEO) with a high molecular mass of 8X10(6) was applied to rinse the capillary to form a dynamic coating which would decrease the undesirable proteins adsorbed to the inner wall of the silica. The electroosmotic flow (EOF) mobility of the five procedures was determined, respectively. It is found that acetonitrile is mainly responsible for the good resolution of PEGylated proteins with the help of PEO coating in the semi-aqueous system. The low EOF mobility and current in the semi-aqueous system might also have some responsibility for the high resolution. The semi-aqueous procedure described in this paper also demonstrates higher resolution of natural proteins than aqueous ones. (C) 2001 Elsevier Science B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000166339600029
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/84611
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Li, WJ,Zhong, Y,Lin, BC,et al. Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY A,2001,905(1-2):299-307.
APA Li, WJ,Zhong, Y,Lin, BC,&Su, ZG.(2001).Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis.JOURNAL OF CHROMATOGRAPHY A,905(1-2),299-307.
MLA Li, WJ,et al."Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis".JOURNAL OF CHROMATOGRAPHY A 905.1-2(2001):299-307.
Files in This Item:
File Name/Size DocType Version Access License
200154MW6F838T.pdf(343KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, WJ]'s Articles
[Zhong, Y]'s Articles
[Lin, BC]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, WJ]'s Articles
[Zhong, Y]'s Articles
[Lin, BC]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, WJ]'s Articles
[Zhong, Y]'s Articles
[Lin, BC]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.