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题名: Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis
作者: Li, WJ;  Zhong, Y;  Lin, BC;  Su, ZG
关键词: semi-aqueous capillary electrophoresis ;  capillary electrophoresis ;  buffer composition ;  proteins ;  poly(ethylene glycol)
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2001-01-05
卷: 905, 期:1-2, 页:299-307
收录类别: SCI
文章类型: Article
部门归属: 18035
项目归属: 1807
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A new program to characterize polyethylene glycol-modified (PEGylated) proteins is outlined using capillary zone electrophoresis (CZE). PEGylated ribonuclease A and lysozyme were selected as examples. Five separation procedures were compared to select out the mixed buffer of acetonitrile-water (1:1, v/v) at pH 2.5 as the best to characterize the PEGylated proteins without sample pretreatment. Polyethylene oxide (PEO) with a high molecular mass of 8X10(6) was applied to rinse the capillary to form a dynamic coating which would decrease the undesirable proteins adsorbed to the inner wall of the silica. The electroosmotic flow (EOF) mobility of the five procedures was determined, respectively. It is found that acetonitrile is mainly responsible for the good resolution of PEGylated proteins with the help of PEO coating in the semi-aqueous system. The low EOF mobility and current in the semi-aqueous system might also have some responsibility for the high resolution. The semi-aqueous procedure described in this paper also demonstrates higher resolution of natural proteins than aqueous ones. (C) 2001 Elsevier Science B.V. All rights reserved.
关键词[WOS]: MODIFIED SUPEROXIDE-DISMUTASE ;  MODIFIED LYSOZYME ;  ASPARAGINASE ;  RIBONUCLEASE ;  ACTIVATION ;  ENZYMES ;  CHARGE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000166339600029
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84611
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Li, WJ,Zhong, Y,Lin, BC,et al. Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY A,2001,905(1-2):299-307.
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