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题名: Electrophoretic migration behavior of DNA fragments in polymer solution
作者: Jin, Y;  Lin, BC;  Fung, YS
关键词: capillary electrophoresis ;  DNA separation ;  polymer coil shrinking theory ;  entangled polymer solution theory
刊名: ELECTROPHORESIS
发表日期: 2001-07-01
卷: 22, 期:11, 页:2150-2158
收录类别: ISTP ;  SCI
文章类型: Article
部门归属: 18038
项目归属: 1807
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A newly developed polymer coil shrinking theory is described and compared with the existing entangled solution theory to explain electrophoretic migration behaviour of DNA in hydroxypropylmethylcellulose (HPMC) polymer solution in buffer containing 100 mM tris(hydroxymethyl)aminomethane 100 mM boric acid, 2 mm ethylenediaminetetraacetic acid at pH 8.3. The polymer coil shrinking theory gave a better model to explain the results obtained. The polymer coil shrinking concentration, C-s, was found to be 0.305% and the uniform entangled concentration, C+, 0.806%. The existence of three regions (the dilute, semidilute, and concentrated solution) at different polymer concentrations enables a better understanding of the system to guide the selection of the best conditions to separate DNA fragments. For separating large fragments (700/800 bp), dilute solutions (HPMC < 0.3%) should be used to achieve a short migration time (10 min). For small fragments (200/300 bp), concentrated solutions are preferred to obtain constant resolution and uniform separation. The best resolution is 0.6% HPMC due to a combined interaction of the polymer coils and the entangled structure. The possibility of DNA separation in semidilute solution is often neglected and the present results indicate that this region has a promising potential for analytical separation of DNA fragments.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  SEPARATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000170175800005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84621
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Hong Kong, Ctr Appl Spect & Analyt Sci, Dept Chem, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Jin, Y,Lin, BC,Fung, YS. Electrophoretic migration behavior of DNA fragments in polymer solution[J]. ELECTROPHORESIS,2001,22(11):2150-2158.
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