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A collision model for DNA separation by capillary electrophoresis in dilute polymer solution
Jin, Y; Lin, BC; Fung, YS
刊名FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY
2001-08-01
370期:8页:1015-1022
收录类别SCI
文章类型Article
部门归属18044
项目归属1807
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]RESTRICTION FRAGMENTS ; GEL-ELECTROPHORESIS ; POLYACRYLAMIDE ; EFFICIENCY ; CELLULOSE
英文摘要A theoretical description. based on chemical kinetics and electrochemistry, is given of DNA separation in dilute polymer solution by capillary electrophoresis. A self-consistent model was developed leading to predictions of the DNA electrophoretic velocity as a function of the experimental conditions - polymer concentration, temperature, and electric field strength. The effect of selected experimental variables is discussed. The phenomena discussed are illustrated for the example of 100 bp DNA ladder separation in dilute HPMC solution by capillary electrophoresis. This model is the first single model that can fully explain the dependence of DNA electrophoretic velocity on electrophoretic conditions.
语种英语
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WOS记录号WOS:000175276300007
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/84639
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China
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GB/T 7714
Jin, Y,Lin, BC,Fung, YS. A collision model for DNA separation by capillary electrophoresis in dilute polymer solution[J]. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY,2001,370(8):1015-1022.
APA Jin, Y,Lin, BC,&Fung, YS.(2001).A collision model for DNA separation by capillary electrophoresis in dilute polymer solution.FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY,370(8),1015-1022.
MLA Jin, Y,et al."A collision model for DNA separation by capillary electrophoresis in dilute polymer solution".FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY 370.8(2001):1015-1022.
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