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题名: A collision model for DNA separation by capillary electrophoresis in dilute polymer solution
作者: Jin, Y;  Lin, BC;  Fung, YS
刊名: FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2001-08-01
卷: 370, 期:8, 页:1015-1022
收录类别: SCI
文章类型: Article
部门归属: 18044
项目归属: 1807
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A theoretical description. based on chemical kinetics and electrochemistry, is given of DNA separation in dilute polymer solution by capillary electrophoresis. A self-consistent model was developed leading to predictions of the DNA electrophoretic velocity as a function of the experimental conditions - polymer concentration, temperature, and electric field strength. The effect of selected experimental variables is discussed. The phenomena discussed are illustrated for the example of 100 bp DNA ladder separation in dilute HPMC solution by capillary electrophoresis. This model is the first single model that can fully explain the dependence of DNA electrophoretic velocity on electrophoretic conditions.
关键词[WOS]: RESTRICTION FRAGMENTS ;  GEL-ELECTROPHORESIS ;  POLYACRYLAMIDE ;  EFFICIENCY ;  CELLULOSE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000175276300007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84639
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Jin, Y,Lin, BC,Fung, YS. A collision model for DNA separation by capillary electrophoresis in dilute polymer solution[J]. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY,2001,370(8):1015-1022.
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