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A collision model for DNA separation by capillary electrophoresis in dilute polymer solution
Jin, Y; Lin, BC; Fung, YS
Source PublicationFRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY
2001-08-01
Volume370Issue:8Pages:1015-1022
Indexed BySCI
SubtypeArticle
Department18044
Funding Project1807
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordRESTRICTION FRAGMENTS ; GEL-ELECTROPHORESIS ; POLYACRYLAMIDE ; EFFICIENCY ; CELLULOSE
AbstractA theoretical description. based on chemical kinetics and electrochemistry, is given of DNA separation in dilute polymer solution by capillary electrophoresis. A self-consistent model was developed leading to predictions of the DNA electrophoretic velocity as a function of the experimental conditions - polymer concentration, temperature, and electric field strength. The effect of selected experimental variables is discussed. The phenomena discussed are illustrated for the example of 100 bp DNA ladder separation in dilute HPMC solution by capillary electrophoresis. This model is the first single model that can fully explain the dependence of DNA electrophoretic velocity on electrophoretic conditions.
Language英语
URL查看原文
WOS IDWOS:000175276300007
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/84639
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Jin, Y,Lin, BC,Fung, YS. A collision model for DNA separation by capillary electrophoresis in dilute polymer solution[J]. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY,2001,370(8):1015-1022.
APA Jin, Y,Lin, BC,&Fung, YS.(2001).A collision model for DNA separation by capillary electrophoresis in dilute polymer solution.FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY,370(8),1015-1022.
MLA Jin, Y,et al."A collision model for DNA separation by capillary electrophoresis in dilute polymer solution".FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY 370.8(2001):1015-1022.
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