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题名: Analysis of urinary 8-hydroxydeoxyguanosine by capillary electrophoresis and solid-phase extraction
作者: Mei, SR;  Xu, GW;  Wu, CY
关键词: 8-hydroxydeoxyguanosine ;  capillary electrophoresis ;  solid-phase extraction ;  urine sample
刊名: ANALYTICAL LETTERS
发表日期: 2001
卷: 34, 期:12, 页:2063-2076
收录类别: SCI
文章类型: Article
部门归属: 18051
项目归属: 1808
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Urinary 8-hydroxydeoxyguanosine (80HdG) has been considered as an excellent marker of individuals at high risk of developing cancer. Until now, urinary 80HdG has largely been measured by high-performance liquid chromatography with electrochemical detection. A new method for the analysis of urinary 80HdG by high-performance capillary electrophoresis has been developed and optimized in our laboratory. A single step solid-phase extraction procedure was optimized and used for extracting 80HdG from human urine. Separations were performed in an uncoated silica capillary (50 cm x 50 tm i.d.) using a P/ACE MDQ system with UV detection. The separation of 80HdG from interfering urinary matrix components is optimized with regard to pH, applied voltage, pressure injection time and concentration of SDS in running buffer. The detection limit of this method is 0.4 mug/ml, the linear range is 0.8-500 mug/ml, the correlation coefficients levels is better than 0.999. The developed method is simple, fast and good reproducibility, furthermore, it requires a very small injection volumes and low costs of analysis, which makes it possible to provide a new noninvasive assay for an indirect measurement of oxidative DNA damage.
关键词[WOS]: OXIDATIVE DNA-DAMAGE ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ELECTROCHEMICAL DETECTION ;  8-HYDROXY-2'-DEOXYGUANOSINE ;  ASSAY ;  BIOMARKER ;  8-HYDROXYGUANINE ;  RESIDUE ;  MARKER ;  BASE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000171530100006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84649
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Mei, SR,Xu, GW,Wu, CY. Analysis of urinary 8-hydroxydeoxyguanosine by capillary electrophoresis and solid-phase extraction[J]. ANALYTICAL LETTERS,2001,34(12):2063-2076.
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