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题名: Simple derivatization method for sensitive determination of fatty acids with fluorescence detection by high-performance liquid chromatography using 9-(2-hydroxyethyl)-carbazole as derivatization reagent
作者: You, JM;  Zhang, WB;  Zhang, YK
关键词: 4-dimethylaminopyridine ;  high-performance liquid chromatography ;  9-(2-hydroxyethyl)-carbazole
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2001-06-01
卷: 436, 期:1, 页:163-172
收录类别: SCI
文章类型: Article
部门归属: 18086
项目归属: 1810
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A simple and sensitive method for the determination of short and long-chain fatty acids using high-performance liquid chromatography with fluorimetric detection has been developed. The fatty acids were derivatized to their corresponding esters with 9-(2-hydroxyethyl)-carbazole (HEC) in acetonitrile at 60 degreesC with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride as a coupling agent in the presence of 4-dimethylaminopyridine (DMAP). A mixture of esters of C-1-C-20 fatty acids was completely separated within 38 min in conjunction with a gradient elution on a reversed-phase C-18 column. The maximum fluorescence emission for the derivatized fatty acids is at 365 nm (lambda (ex) 335 nm). Studies on derivatization conditions indicate that fatty acids react proceeded rapidly and smoothly with HEC in the presence of EDC and DMAP in acetonitrile to give the corresponding sensitively fluorescent derivatives. The application of this method to the analysis of long chain fatty acids in plasma is also investigated. The LC separation shows good selectivity and reproducibility for fatty acids derivatives. The R.S.D. (n = 6) for each fatty acid derivative are <4%. The detection limits are at 45-68 fmol levels for C-14-C-20 fatty acids and even lower levels for
关键词[WOS]: CARBOXYLIC-ACIDS ;  LABELING REAGENT ;  PRECOLUMN DERIVATIZATION ;  FLUOROMETRIC DETECTION ;  AMINO-ACIDS ;  DERIVATIVES ;  TRIFLUOROMETHANESULFONATE ;  9-ANTHRYLDIAZOMETHANE ;  PROSTAGLANDINS ;  CHLORIDE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000169173000018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/84661
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
You, JM,Zhang, WB,Zhang, YK. Simple derivatization method for sensitive determination of fatty acids with fluorescence detection by high-performance liquid chromatography using 9-(2-hydroxyethyl)-carbazole as derivatization reagent[J]. ANALYTICA CHIMICA ACTA,2001,436(1):163-172.
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